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The bio-response of osteocytes and its regulation on osteoblasts under vibration.
Wu, Xin-Tong; Sun, Lian-Wen; Qi, Hong-Yu; Shi, Hao; Fan, Yu-Bo.
Afiliación
  • Wu XT; Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, 37th Xueyuan Road, Hian-dian District, Beijing, China.
  • Sun LW; Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, 37th Xueyuan Road, Hian-dian District, Beijing, China.
  • Qi HY; International Joint Research Center of Aerospace Biotechnology and Medical Engineering, Ministry of Science and Technology of China, Beijing, China.
  • Shi H; School of Energy and Power Engineering, Beihang University, 37th Xueyuan Road, Hian-dian District, Beijing, China.
  • Fan YB; School of Energy and Power Engineering, Beihang University, 37th Xueyuan Road, Hian-dian District, Beijing, China.
Cell Biol Int ; 40(4): 397-406, 2016 Apr.
Article en En | MEDLINE | ID: mdl-26715381
Vibration, especially at low magnitude and high frequency (LMHF), was demonstrated to be anabolic for bone, but how the LMHF vibration signal is perceived by osteocytes is not fully studied. On the other hand, the mechanotransduction of osteocytes under shear stress has been scientists' primary focus for years. Due to the small strain caused by low-magnitude vibration, whether the previous explanation for shear stress will still work for LMHF vibration is unknown. In this study, a finite element method (FEM) model based on the real geometrical shape of an osteocyte was built to compare the mechanical behaviors of osteocytes under LMHF vibration and shear stress. The bio-response of osteocytes to vibration under different frequencies, including the secretion of soluble factors and the concentration of intracellular calcium, were studied. The regulating effect of the conditioned medium (CM) from vibrated osteocytes on osteoblasts was also studied. The FEM analysis result showed the cell membrane deformation under LMHF vibration was very small (with a peak value of 1.09%) as compared to the deformation caused by shear stress (with a peak value of 6.65%). The F-actin stress fibers of osteocytes were reorganized, especially on the nucleus periphery after LMHF vibration. The vibration at 30 Hz has a promoting effect on osteocytes and the osteogenesis of osteoblasts, whereas vibration at 90 Hz was suppressive. These results lead to a conclusion that the bio-response of osteocytes to LMHF vibration is frequency-dependent and is more related to the cytoskeleton on nuclear periphery rather than the membrane deformation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteocitos / Vibración Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cell Biol Int Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteocitos / Vibración Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cell Biol Int Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido