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[Identification of Bx02 Alleles and Genetic Analysis of the Involved Ancestry].
Liu, Guo-Liang; Zhu, Fang; Zheng, Xiao-Li; Cao, Cheng-Hua; He, Ya-Jing; Cheng, Xiang-Shu; Li, Tao; Ji, Xin-Ying.
Afiliación
  • Liu GL; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
  • Zhu F; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
  • Zheng XL; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
  • Cao CH; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
  • He YJ; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
  • Cheng XS; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
  • Li T; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
  • Ji XY; Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China. E-mail: xinying_ji@henu.edu.cn.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 23(6): 1749-52, 2015 Dec.
Article en Zh | MEDLINE | ID: mdl-26708904
OBJECTIVE: To identify the genotypes of the blood sample whose blood grouping showed discrepancies and study the ABO alleles' molecular characteristics of the involved ancestry. METHODS: Blood samples were preliminary genotyped by PCR-SSP. Complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and cloning sequenced to identify its genotype. RESULTS: Sequence analysis indicated that 3 samples of the family had an nt905A>G mutation in the B gene compared with ABO*B101. Combined with the serological results, the propositus could be typed as Bx02/O102. CONCLUSION: DNA sequencing analysis is able to identify the serological phenotype samples that forward and reverse group methods were incongruous.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alelos Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: Zh Revista: Zhongguo Shi Yan Xue Ye Xue Za Zhi Asunto de la revista: HEMATOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alelos Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: Zh Revista: Zhongguo Shi Yan Xue Ye Xue Za Zhi Asunto de la revista: HEMATOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: China Pais de publicación: China