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Early changes in system [Formula: see text] and glutathione in the retina of diabetic rats.
Carpi-Santos, Raul; Ferreira, Marcos José; Pereira Netto, Annibal Duarte; Giestal-de-Araujo, Elizabeth; Ventura, Ana Lucia Marques; Cossenza, Marcelo; Calaza, Karin C.
Afiliación
  • Carpi-Santos R; Department of Neurobiology, Neuroscience Program, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil.
  • Ferreira MJ; Department of Analytical Chemistry, Institute of Chemistry, Fluminense Federal University, Niterói, RJ, Brazil.
  • Pereira Netto AD; Department of Analytical Chemistry, Institute of Chemistry, Fluminense Federal University, Niterói, RJ, Brazil.
  • Giestal-de-Araujo E; Department of Neurobiology, Neuroscience Program, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil.
  • Ventura ALM; Department of Neurobiology, Neuroscience Program, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil.
  • Cossenza M; Department of Physiology and Pharmacology, Neuroscience Program, Biomedical Institute, Fluminense Federal University, Niterói, RJ, Brazil.
  • Calaza KC; Department of Neurobiology, Neuroscience Program, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil. Electronic address: karincalaza@gmail.com.
Exp Eye Res ; 146: 35-42, 2016 05.
Article en En | MEDLINE | ID: mdl-26706282
Diabetic retinopathy (DR), the main cause of blindness among diabetic patients, affects both neuronal and vascular cells of the retina. Studies show that neuronal cell death begins after 4 weeks of diabetes and could be related with an increase in oxidative stress. System [Formula: see text] is a glutamate/cystine exchanger, formed by a catalytic subunit called xCT and a regulatory subunit 4F2hc, whose activity is crucial to the synthesis of glutathione, which is a key antioxidant molecule for cells. Although some studies have shown that glutamate transport mediated by excitatory amino acid transporters (EAATs) in diabetic rats is downregulated, there are no studies investigating system [Formula: see text] in this context. To evaluate whether system [Formula: see text] is modified by early onset of diabetes, primary retinal cell culture exposed to high glucose and retinas of rats 3 weeks after streptozotocin injection were used. We observed that xCT subunit protein expression both in cultures and in vivo were diminished. Furthermore, system [Formula: see text] activity and GSH levels were also decreased whereas oxidative stress was increased in retinas of diabetic animals. Therefore, this study raises the possibility that alterations in system [Formula: see text] expression and activity could occur during early onset of diabetes. In that way, system [Formula: see text] modifications could be related to increased ROS in diabetic retinopathy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Especies Reactivas de Oxígeno / Estrés Oxidativo / Diabetes Mellitus Experimental / Retinopatía Diabética / Glutatión Límite: Animals Idioma: En Revista: Exp Eye Res Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Especies Reactivas de Oxígeno / Estrés Oxidativo / Diabetes Mellitus Experimental / Retinopatía Diabética / Glutatión Límite: Animals Idioma: En Revista: Exp Eye Res Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido