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Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line.
Demir, Selim; Aliyazicioglu, Yuksel; Turan, Ibrahim; Misir, Sema; Mentese, Ahmet; Yaman, Serap Ozer; Akbulut, Kubra; Kilinc, Kagan; Deger, Orhan.
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  • Demir S; a Department of Medical Biochemistry , Institute of Health Sciences, Karadeniz Technical University , Trabzon , Turkey.
  • Aliyazicioglu Y; b Department of Medical Biochemistry , Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey.
  • Turan I; c Department of Genetic and Bioengineering , Faculty of Engineering and Natural Sciences, Gumushane University, Gumushane, Turkey, and Medicinal Plants, Traditional Medicine Practice and Research Center, Gumushane University , Gumushane , Turkey.
  • Misir S; d Department of Medical Biochemistry , Institute of Health Sciences, Karadeniz Technical University, Trabzon, Turkey and Department of Biochemistry, Faculty of Pharmacy, Cumhuriyet University , Sivas , Turkey.
  • Mentese A; b Department of Medical Biochemistry , Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey.
  • Yaman SO; a Department of Medical Biochemistry , Institute of Health Sciences, Karadeniz Technical University , Trabzon , Turkey.
  • Akbulut K; a Department of Medical Biochemistry , Institute of Health Sciences, Karadeniz Technical University , Trabzon , Turkey.
  • Kilinc K; e Department of Genetic and Bioengineering , Faculty of Engineering and Natural Sciences, Gumushane University , Gumushane , Turkey.
  • Deger O; b Department of Medical Biochemistry , Faculty of Medicine, Karadeniz Technical University , Trabzon , Turkey.
Nutr Cancer ; 68(1): 165-72, 2016.
Article en En | MEDLINE | ID: mdl-26700423
Cancer is a heterogeneous disease, two of whose characteristic features are uncontrollable cell proliferation and insufficient apoptosis. Various studies have investigated the antiproliferative effects of propolis, a natural bee product, from different countries, and its cytotoxic effects have been attributed to its polyphenol contents. The purpose of this study was to show the cytotoxic effects, and possible mechanisms involved, of ethanolic extract of Turkish propolis (EEP) on the human lung cancer (A549) cell line. Cytotoxic activity of EEP on A549 cells was revealed using the MTT assay. Mechanisms involved in the cytotoxic action of EEP on A549 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, endoplasmic reticulum stress using RT-PCR, and caspase activity using luminometric analysis. EEP exhibited selective toxicity against A549 cells compared to normal fibroblast cells. We determined that EEP arrested the cell cycle of A549 cells at the G1 phase, induced endoplasmic reticulum stress, caspase activity, and apoptosis and reduced mitochondrial membrane potential. These results indicate that Turkish propolis is capable of reducing cancer cell proliferation and may have a promising role to play in the development of new anticancer drugs in the future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Própolis / Apoptosis / Neoplasias Pulmonares / Antineoplásicos Límite: Humans Idioma: En Revista: Nutr Cancer Año: 2016 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Própolis / Apoptosis / Neoplasias Pulmonares / Antineoplásicos Límite: Humans Idioma: En Revista: Nutr Cancer Año: 2016 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos