Your browser doesn't support javascript.
loading
Hair cell regeneration or the expression of related factors that regulate the fate specification of supporting cells in the cochlear ducts of embryonic and posthatch chickens.
Jiang, Lingling; Jin, Ran; Xu, Jincao; Ji, Yubin; Zhang, Meiguang; Zhang, Xuebo; Zhang, Xinwen; Han, Zhongming; Zeng, Shaoju.
Afiliación
  • Jiang L; Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, China.
  • Jin R; Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, China.
  • Xu J; Department of Otorhinolaryngology, General Hospital of the Second Artillery, PLA, Beijing, China.
  • Ji Y; Department of Otorhinolaryngology, General Hospital of the Second Artillery, PLA, Beijing, China.
  • Zhang M; Department of Otorhinolaryngology, General Hospital of the Second Artillery, PLA, Beijing, China.
  • Zhang X; College of Life Sciences, Hainan Normal University, Haikou, China.
  • Zhang X; College of Life Sciences, Hainan Normal University, Haikou, China.
  • Han Z; Department of Otorhinolaryngology, General Hospital of the Second Artillery, PLA, Beijing, China. Electronic address: zhongminghan@yahoo.com.cn.
  • Zeng S; Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, China. Electronic address: sjzeng@bnu.edu.cn.
Hear Res ; 332: 17-28, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26688175
Hair cells in posthatch chickens regenerate spontaneously through mitosis or the transdifferentiation of supporting cells in response to antibiotic injury. However, how embryonic chicken cochleae respond to antibiotic treatment remains unknown. This study is the first to indicate that unlike hair cells in posthatch chickens, the auditory epithelium was free from antibiotic injury (25-250 mg gentamicin/kg) in embryonic chickens, although FITC-conjugated gentamicin actually reached embryonic hair cells. Next, we examined and counted the cells and performed labeling for BrdU, Sox2, Atoh1/Math1, PV or p27(kip1) (triple or double labeling) in the injured cochlea ducts after gentamicin treatment at 2 h (h), 15 h, 24 h, 2 days (d), 3 d and 7 d after BrdU treatment in posthatch chickens. Our results indicated that following gentamicin administration, proliferating cells (BrdU+) were labeled for Atoh1/Math1 in the damaged areas 3d after gentamicin administration, whereas hair cells (PV+) renewed through mitosis (BrdU+) or direct transdifferentiation (BrdU-) were evident only after 5 d of gentamicin administration. In addition, Sox2 expression was up-regulated in triggered supporting cells at an early stage of regeneration, but stopped at the advent of mature hair cells. Our study also indicated that p27(kip1) was expressed in both hair cells and supporting cells but was down-regulated in a subgroup of the supporting cells that gave rise to hair cells. These data and the obtained dynamic changes of the cells labeled for BrdU, Sox2, Atoh1/Math1, PV or p27(kip1) are useful for understanding supporting cell behaviors and their fate specification during hair cell regeneration.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Gentamicinas / Conducto Coclear / Linaje de la Célula / Transdiferenciación Celular / Células Ciliadas Auditivas / Células Laberínticas de Soporte / Antibacterianos Límite: Animals Idioma: En Revista: Hear Res Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Gentamicinas / Conducto Coclear / Linaje de la Célula / Transdiferenciación Celular / Células Ciliadas Auditivas / Células Laberínticas de Soporte / Antibacterianos Límite: Animals Idioma: En Revista: Hear Res Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos