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Detection of intestinal parasites by use of the cuvette-based automated microscopy analyser sediMAX(®).
Intra, J; Taverna, E; Sala, M R; Falbo, R; Cappellini, F; Brambilla, P.
Afiliación
  • Intra J; Department of Laboratory Medicine, University Milano Bicocca, Desio Hospital, Desio, Italy. Electronic address: jari.intra@unimi.it.
  • Taverna E; Department of Laboratory Medicine, University Milano Bicocca, Desio Hospital, Desio, Italy.
  • Sala MR; Department of Laboratory Medicine, University Milano Bicocca, Desio Hospital, Desio, Italy.
  • Falbo R; Department of Laboratory Medicine, University Milano Bicocca, Desio Hospital, Desio, Italy.
  • Cappellini F; Department of Laboratory Medicine, University Milano Bicocca, Desio Hospital, Desio, Italy.
  • Brambilla P; Department of Laboratory Medicine, University Milano Bicocca, Desio Hospital, Desio, Italy.
Clin Microbiol Infect ; 22(3): 279-84, 2016 Mar.
Article en En | MEDLINE | ID: mdl-26679923
Microscopy is the reference method for intestinal parasite identification. The cuvette-based automated microscopy analyser, sediMAX 1, provides 15 digital images of each sediment sample. In this study, we have evaluated this fully automated instrument for detection of enteric parasites, helminths and protozoa. A total of 700 consecutively preserved samples consisting of 60 positive samples (50 protozoa, ten helminths) and 640 negative samples were analysed. Operators were blinded to each others' results. Samples were randomized and were tested both by manual microscopy and sediMAX 1 for parasite recognition. The sediMAX 1 analysis was conducted using a dilution of faecal samples, allowing determination of morphology. The data obtained using sediMAX 1 showed a specificity of 100% and a sensitivity of 100%. Some species of helminths, such as Enterobius vermicularis, Strongyloides stercolaris, the Ancylostoma duodenale/Necator americanus complex, and schistosomes were not considered in this work, because they are rare in stool specimens, are not easily detectable with microscopy analysis, and require specific recovery techniques. This study demonstrated for the first time that sediMAX 1 can be an aid in enteric parasite identification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Automatización / Parasitosis Intestinales / Microscopía Tipo de estudio: Clinical_trials / Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Clin Microbiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Automatización / Parasitosis Intestinales / Microscopía Tipo de estudio: Clinical_trials / Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Clin Microbiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido