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T7 replisome directly overcomes DNA damage.
Sun, Bo; Pandey, Manjula; Inman, James T; Yang, Yi; Kashlev, Mikhail; Patel, Smita S; Wang, Michelle D.
Afiliación
  • Sun B; Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.
  • Pandey M; Howard Hughes Medical Institute, Cornell University, Ithaca, New York 14853, USA.
  • Inman JT; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Yang Y; Department of Biochemistry and Molecular Biology, Rutgers-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
  • Kashlev M; Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.
  • Patel SS; Howard Hughes Medical Institute, Cornell University, Ithaca, New York 14853, USA.
  • Wang MD; Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.
Nat Commun ; 6: 10260, 2015 Dec 17.
Article en En | MEDLINE | ID: mdl-26675048
Cells and viruses possess several known 'restart' pathways to overcome lesions during DNA replication. However, these 'bypass' pathways leave a gap in replicated DNA or require recruitment of accessory proteins, resulting in significant delays to fork movement or even cell division arrest. Using single-molecule and ensemble methods, we demonstrate that the bacteriophage T7 replisome is able to directly replicate through a leading-strand cyclobutane pyrimidine dimer (CPD) lesion. We show that when a replisome encounters the lesion, a substantial fraction of DNA polymerase (DNAP) and helicase stay together at the lesion, the replisome does not dissociate and the helicase does not move forward on its own. The DNAP is able to directly replicate through the lesion by working in conjunction with helicase through specific helicase-DNAP interactions. These observations suggest that the T7 replisome is fundamentally permissive of DNA lesions via pathways that do not require fork adjustment or replisome reassembly.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dímeros de Pirimidina / Daño del ADN / ADN Viral / Bacteriófago T7 / ADN Helicasas / ADN Polimerasa Dirigida por ADN / Replicación del ADN Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dímeros de Pirimidina / Daño del ADN / ADN Viral / Bacteriófago T7 / ADN Helicasas / ADN Polimerasa Dirigida por ADN / Replicación del ADN Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido