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Carbendazim exposure induces developmental, biochemical and behavioural disturbance in zebrafish embryos.
Andrade, Thayres S; Henriques, Jorge F; Almeida, Ana Rita; Machado, Ana Luísa; Koba, Olga; Giang, Pham Thai; Soares, Amadeu M V M; Domingues, Inês.
Afiliación
  • Andrade TS; Dept. of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. Electronic address: thayres@gmail.com.
  • Henriques JF; Dept. of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
  • Almeida AR; Dept. of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
  • Machado AL; Dept. of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
  • Koba O; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.
  • Giang PT; University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, Vodnany 389 25, Czech Republic.
  • Soares AMVM; Dept. of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
  • Domingues I; Dept. of Biology and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Aquat Toxicol ; 170: 390-399, 2016 Jan.
Article en En | MEDLINE | ID: mdl-26653011
Carbendazim is a widely used broad spectrum benzimidazole fungicide; however, its effects to non-target aquatic organisms are poorly studied. The aim of this study was to investigate the toxic effects of carbendazim to zebrafish early life stages at several levels of biological organization, including developmental, biochemical and behavioural levels. The embryo assay was done following the OECD guideline 236 and using a concentration range between 1.1 and 1.8mg/L. Lethal and developmental endpoints such as hatching, edemas, malformations, heart beat rate, body growth and delays were assessed in a 96h exposure. A sub-teratogenic range (from 0.16 to 500µg/L) was then used to assess effects at biochemical and behavioural levels. Biochemical markers included cholinesterase (ChE), glutathione-S-transferase (GST), lactate dehydrogenase (LDH) and catalase (CAT) and were assessed at 96h. The locomotor behaviour was assessed using an automated video tracking system at 120h. Carbendazim (96h-LC50 of 1.75mg/L) elicited several developmental anomalies in zebrafish embryos with EC50 values ranging from 0.85 to 1.6mg/L. ChE, GST and LDH activities were increased at concentrations equal or above 4µg/L. The locomotor assay showed to be extremely sensitive, detecting effects in time that larvae spent swimming at concentrations of 0.16µg/L and thus, being several orders of magnitude more sensitive that developmental parameters or lethality. These are ecological relevant concentrations and highlight the potential of behavioural endpoints as early warning signs for environmental stress. Further studies should focus on understanding how the behavioural disturbances measured in these types of studies translate into fitness impairment at the adult stage.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Conducta Animal / Bencimidazoles / Pez Cebra / Carbamatos / Fungicidas Industriales Límite: Animals Idioma: En Revista: Aquat Toxicol Asunto de la revista: BIOLOGIA / TOXICOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Conducta Animal / Bencimidazoles / Pez Cebra / Carbamatos / Fungicidas Industriales Límite: Animals Idioma: En Revista: Aquat Toxicol Asunto de la revista: BIOLOGIA / TOXICOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos