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SVCT2 Overexpression in Neuroblastoma Cells Induces Cellular Branching that is Associated with ERK Signaling.
Salazar, Katterine; Martínez, Milka; Ulloa, Viviana; Bertinat, Romina; Martínez, Fernando; Jara, Nery; Espinoza, Francisca; Bongarzone, Ernesto R; Nualart, Francisco.
Afiliación
  • Salazar K; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
  • Martínez M; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
  • Ulloa V; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
  • Bertinat R; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
  • Martínez F; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
  • Jara N; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
  • Espinoza F; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile.
  • Bongarzone ER; Department of Anatomy and Cell Biology, College of Medicine, University of Illinois Chicago, Chicago, IL, USA.
  • Nualart F; Laboratory of Neurobiology and Stem Cells, Center for Advanced Microscopy CMA BIO BIO, Faculty of Biological Sciences, University of Concepcion, Concepción, Chile. frnualart@udec.cl.
Mol Neurobiol ; 53(10): 6668-6679, 2016 12.
Article en En | MEDLINE | ID: mdl-26646539
Expression of the sodium and ascorbic acid (AA) cotransporter SVCT2 is induced during the period of cellular arborization and synaptic maturation of early postnatal (P1-P5) rat cerebral neurons. The physiological importance of the transporter for neurons is evidenced by the lethality and delayed neuronal differentiation detected in mice with ablation of SVCT2. The mechanism(s) involved in these defects and the role of SVCT2 in neuronal branching have not been determined yet. To address this, we used lentiviral expression vectors to increase the levels of SVCT2 in N2a cells and analyzed the effects on neurite formation. Expression of a fusion protein containing the human SVCT2wt and EYFP induced an increase in the number of MAP2+ neurites and filopodia in N2a cells. Overexpression of SVCT2 and treatment with AA promoted ERK1/2 phosphorylation. Our data suggest that enhanced expression of the high affinity AA transporter SVCT2, which tightly regulates intracellular AA concentrations, induces neuronal branching that then activates key signaling pathways that are involved in the differentiation and maturation of cortical neurons during postnatal development.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema de Señalización de MAP Quinasas / Transportadores de Sodio Acoplados a la Vitamina C / Neuroblastoma Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Mol Neurobiol Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema de Señalización de MAP Quinasas / Transportadores de Sodio Acoplados a la Vitamina C / Neuroblastoma Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Mol Neurobiol Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Estados Unidos