Simple method for precise determination of chemical lethality in the L-arabinose resistance test of Salmonella typhimurium.
Mutat Res
; 226(3): 175-80, 1989 Jul.
Article
en En
| MEDLINE
| ID: mdl-2664500
A simple method is described for the determination of the lethal effects of chemicals assayed with the L-arabinose resistance test of Salmonella typhimurium. The method uses a mixed culture of 2 isogenic bacterial strains which are distinguished on the basis of their different nutritional requirements: sensitivity or resistance to L-arabinose, auxotrophy or prototrophy to histidine and leucine. BA13 (the mutation indicator strain) is the strain for routine screening of mutagens and allows the selection of forward mutation from L-arabinose sensitivity to L-arabinose resistance. BAL13 (the survival indicator strain) is a derivative of BA13. Both bacterial strains are found to be equally sensitive to the lethal effects of mutagens. The method described permits the measurement of cell survival at the same high cell concentration as used in the measurement of the mutant yield and in the same type of minimal medium with L-arabinose and glycerol, except for the histidine supplement in the mutant plates or the leucine in the survival plates.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arabinosa
/
Salmonella typhimurium
/
Pruebas de Mutagenicidad
/
Mutágenos
Idioma:
En
Revista:
Mutat Res
Año:
1989
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Países Bajos