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Expression pattern of the Hedgehog signaling pathway in pituitary adenomas.
Yavropoulou, Maria P; Maladaki, Anna; Topouridou, Konstantina; Kotoula, Vasiliki; Poulios, Chris; Daskalaki, Emily; Foroglou, Nikolaos; Karkavelas, George; Yovos, John G.
Afiliación
  • Yavropoulou MP; Laboratory of Molecular and Clinical Endocrinology, 1st Department of Internal Medicine, AHEPA Univ. Hospital, Aristotle University of Thessaloniki, Greece. Electronic address: margia@med.auth.gr.
  • Maladaki A; Laboratory of Molecular and Clinical Endocrinology, 1st Department of Internal Medicine, AHEPA Univ. Hospital, Aristotle University of Thessaloniki, Greece.
  • Topouridou K; Laboratory of Molecular and Clinical Endocrinology, 1st Department of Internal Medicine, AHEPA Univ. Hospital, Aristotle University of Thessaloniki, Greece.
  • Kotoula V; Pathology Department, Faculty of Medicine, Aristotle University of Thessaloniki, Greece.
  • Poulios C; Pathology Department, Faculty of Medicine, Aristotle University of Thessaloniki, Greece.
  • Daskalaki E; Pathology Department, Faculty of Medicine, Aristotle University of Thessaloniki, Greece.
  • Foroglou N; Department of Neurosurgery, AHEPA Univ. Hospital, Aristotle University of Thessaloniki, Greece.
  • Karkavelas G; Pathology Department, Faculty of Medicine, Aristotle University of Thessaloniki, Greece.
  • Yovos JG; Laboratory of Molecular and Clinical Endocrinology, 1st Department of Internal Medicine, AHEPA Univ. Hospital, Aristotle University of Thessaloniki, Greece.
Neurosci Lett ; 611: 94-100, 2016 Jan 12.
Article en En | MEDLINE | ID: mdl-26620835
Several studies have demonstrated the role of Wnt and Notch signaling in the pathogenesis of pituitary adenomas, but data are scarce regarding the role of Hedgehog signaling. In this study we investigated the differential expression of gene targets of the Hedgehog signaling pathway. Formalin-fixed, paraffin-embedded specimens from adult patients who underwent transphenoidal resection and normal human pituitary tissues that were obtained from autopsies were used. Clinical information and data from pre-operative MRI scan (extracellular tumor extension, tumor size, displacement of the optic chiasm) were retrieved from the Hospital's database. We used a customized RT(2) Profiler PCR Array, to investigate the expression of genes related to Notch and Hedgehog signaling pathways (PTCH1, PTCH2, GLI1, GLI3, NOTCH3, JAG1, HES1, and HIP). A total of 52 pituitary adenomas (32 non-functioning adenomas, 15 somatotropinomas and 5 prolactinomas) were used in the final analysis. In non-functioning pituitary adenomas there was a significant decrease (approximately 75%) in expression of all Hedgehog related genes that were tested, while Notch3 and Jagged-1 expression was found significantly increased, compared with normal pituitary tissue controls. In contrast, somatotropinomas demonstrated a significant increase in expression of all Hedgehog related genes and a decrease in the expression of Notch3 and Jagged-1. There was no significant difference in the expression of Hedgehog and Notch related genes between prolactinomas and healthy pituitary tissues. Hedgehog signalling appears to be activated in somatotropinomas but not in non-functioning pituitary adenomas in contrast to the expression pattern of Notch signalling pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Hipofisarias / Adenoma / Proteínas Hedgehog Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Neurosci Lett Año: 2016 Tipo del documento: Article Pais de publicación: Irlanda
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Hipofisarias / Adenoma / Proteínas Hedgehog Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Neurosci Lett Año: 2016 Tipo del documento: Article Pais de publicación: Irlanda