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Novel highly specific anti-periostin antibodies uncover the functional importance of the fascilin 1-1 domain and highlight preferential expression of periostin in aggressive breast cancer.
Field, Sarah; Uyttenhove, Catherine; Stroobant, Vincent; Cheou, Paméla; Donckers, Dominique; Coutelier, Jean-Paul; Simpson, Peter T; Cummings, Margaret C; Saunus, Jodi M; Reid, Lynne E; Kutasovic, Jamie R; McNicol, Anne Marie; Kim, Ba Reun; Kim, Jae Ho; Lakhani, Sunil R; Neville, A Munro; Van Snick, Jacques; Jat, Parmjit S.
Afiliación
  • Field S; University of Oxford Branch, Ludwig Cancer Research, Oxford, United Kingdom.
  • Uyttenhove C; Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen Square, London, United Kingdom.
  • Stroobant V; Ludwig Cancer Research, Brussels Branch, Brussels, Belgium.
  • Cheou P; de Duve Institute, Université Catholique De Louvain, Brussels, Belgium.
  • Donckers D; Ludwig Cancer Research, Brussels Branch, Brussels, Belgium.
  • Coutelier JP; de Duve Institute, Université Catholique De Louvain, Brussels, Belgium.
  • Simpson PT; de Duve Institute, Université Catholique De Louvain, Brussels, Belgium.
  • Cummings MC; de Duve Institute, Université Catholique De Louvain, Brussels, Belgium.
  • Saunus JM; The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, Australia.
  • Reid LE; Cancer Genetics Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Herston, Australia.
  • Kutasovic JR; The University of Queensland, School of Medicine, Discipline of Molecular & Cellular Pathology, Herston, Brisbane, Australia.
  • McNicol AM; The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, Australia.
  • Kim BR; The University of Queensland, School of Medicine, Discipline of Molecular & Cellular Pathology, Herston, Brisbane, Australia.
  • Kim JH; Pathology Queensland, The Royal Brisbane & Women's Hospital, Brisbane, Australia.
  • Lakhani SR; The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, Australia.
  • Neville AM; Cancer Genetics Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Herston, Australia.
  • Van Snick J; The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, Australia.
  • Jat PS; Cancer Genetics Laboratory, QIMR Berghofer Medical Research Institute, Queensland, Herston, Australia.
Int J Cancer ; 138(8): 1959-70, 2016 Apr 15.
Article en En | MEDLINE | ID: mdl-26619948
Periostin (POSTN), a secreted homodimeric protein that binds integrins αvß3, αvß5, and α6ß4, was originally found to be expressed in fetal tissues and in the adult upon injury particularly bone fractures due to its role in remodelling and repair. Recently it was found to be over-expressed in human breast cancer and a variety of other tumour types including head and neck squamous cell carcinoma, where its overexpression correlates with increased tumour invasion. Progress in studying its functional role in tumour pathogenesis has been hampered by the paucity of antibodies for its specific and sensitive detection. It has proven very difficult to obtain monoclonal antibodies (mAbs) against this highly conserved protein but we report here that combining infection of mice with lactate dehydrogenase elevating virus (LDV), a B cell activating arterivirus, with conjugation of human POSTN to ovalbumin as an immunogenic carrier, enabled us to develop six mAbs recognizing both human and mouse POSTN and inhibiting its binding to αvß3 integrin. Two of the mAbs, MPB4B1 and MPC5B4, were tested and found to inhibit POSTN-induced migration of human endothelial colony forming cells. All six mAbs recognized amino acids 136-51 (APSNEAWDNLDSDIRR) within the POSTN fascilin (FAS) 1-1 domain revealing the functional importance of this motif; this was further highlighted by the ability of aa 136-151 peptide to inhibit integrin-mediated cell migration. Immunohistochemistry using MPC5B4, indicated that breast tumour cell POSTN expression was a strong prognostic indicator, along with tumour size, lymph node, and human epidermal growth factor receptor 2 (HER2) status.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Biomarcadores de Tumor / Moléculas de Adhesión Celular / Anticuerpos Monoclonales Tipo de estudio: Prognostic_studies Límite: Adult / Aged / Aged80 / Animals / Female / Humans / Middle aged Idioma: En Revista: Int J Cancer Año: 2016 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Biomarcadores de Tumor / Moléculas de Adhesión Celular / Anticuerpos Monoclonales Tipo de estudio: Prognostic_studies Límite: Adult / Aged / Aged80 / Animals / Female / Humans / Middle aged Idioma: En Revista: Int J Cancer Año: 2016 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos