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High-speed DNA-based rolling motors powered by RNase H.
Yehl, Kevin; Mugler, Andrew; Vivek, Skanda; Liu, Yang; Zhang, Yun; Fan, Mengzhen; Weeks, Eric R; Salaita, Khalid.
Afiliación
  • Yehl K; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322, USA.
  • Mugler A; Department of Physics, Emory University, 400 Dowman Drive, Atlanta, Georgia 30322, USA.
  • Vivek S; Department of Physics, Purdue University, West Lafayette, Indiana 47907, USA.
  • Liu Y; Department of Physics, Emory University, 400 Dowman Drive, Atlanta, Georgia 30322, USA.
  • Zhang Y; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322, USA.
  • Fan M; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322, USA.
  • Weeks ER; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322, USA.
  • Salaita K; Department of Physics, Emory University, 400 Dowman Drive, Atlanta, Georgia 30322, USA.
Nat Nanotechnol ; 11(2): 184-90, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26619152
DNA-based machines that walk by converting chemical energy into controlled motion could be of use in applications such as next-generation sensors, drug-delivery platforms and biological computing. Despite their exquisite programmability, DNA-based walkers are challenging to work with because of their low fidelity and slow rates (∼1 nm min(-1)). Here we report DNA-based machines that roll rather than walk, and consequently have a maximum speed and processivity that is three orders of magnitude greater than the maximum for conventional DNA motors. The motors are made from DNA-coated spherical particles that hybridize to a surface modified with complementary RNA; the motion is achieved through the addition of RNase H, which selectively hydrolyses the hybridized RNA. The spherical motors can move in a self-avoiding manner, and anisotropic particles, such as dimerized or rod-shaped particles, can travel linearly without a track or external force. We also show that the motors can be used to detect single nucleotide polymorphism by measuring particle displacement using a smartphone camera.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Ribonucleasa H / Proteínas Motoras Moleculares / Nanotecnología Idioma: En Revista: Nat Nanotechnol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Ribonucleasa H / Proteínas Motoras Moleculares / Nanotecnología Idioma: En Revista: Nat Nanotechnol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido