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Role of eukaryotic translation initiation factors 3a in hypoxia-induced right ventricular remodeling of rats.
Li, Wen-Qun; Li, Xiao-Hui; Wu, Yue-Han; Du, Jie; Wang, Ai-Ping; Li, Dai; Li, Yuan-Jian.
Afiliación
  • Li WQ; Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.
  • Li XH; Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.
  • Wu YH; Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.
  • Du J; Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China.
  • Wang AP; Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China; Department of Anatomy, School of Medicine, University of South China, Hengyang 421001, China.
  • Li D; National Institution of Drug Clinical Trial, Xiangya Hospital, Central South University, Changsha 410078, China.
  • Li YJ; Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China. Electronic address: yuan_jianli@yahoo.com.
Life Sci ; 144: 61-8, 2016 Jan 01.
Article en En | MEDLINE | ID: mdl-26612348
AIM: Eukaryotic translation initiation factors 3a (eIF3a) is involved in regulating cell cycle, cell division, growth and differentiation. Previous studies suggest a role of eIF3a on fibrosis disease and cellular proliferation and differentiation of fibroblasts. The present study aims to investigate the role of eIF3a on hypoxia-induced right ventricular (RV) remodeling and underlying mechanism. MAIN METHODS: RV remodeling was induced by hypoxia (10% O2, 3 weeks) in rats. Primary cardiac fibroblasts were cultured in vitro and their proliferation was investigated by MTS and EdU incorporation method. eIF3a knockdown was conducted by eIF3a siRNA. The expression/level of TGF-ß1, eIF3a, p27 and α-SMA, collagen-I, collagen-III, ANP and BNP were analyzed by ELISA, real-time PCR or Western blot. KEY FINDINGS: The expression of eIF3a was obviously increased in right ventricle of RV remodeling rats accompanied by up-regulation of α-SMA and collagens. In cultured cardiac fibroblasts, application of exogenous TGF-ß1-induced cellular proliferation and differentiation concomitantly with up-regulation of eIF3a expression and down-regulation of p27 expression. The effects of TGF-ß1-induced proliferation and up-regulation of α-SMA and collagen in cardiac fibroblasts were abolished by eIF3a siRNA. eIF3a siRNA reversed TGF-ß1 induced down-regulation of p27 expression. SIGNIFICANCE: The eIF3a plays a crucial role in hypoxia-induced RV remodeling by regulating TGF-ß1-induced proliferation and differentiation of cardiac fibroblasts, which is mediated via eIF3a/p27 pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipertrofia Ventricular Derecha / Remodelación Ventricular / Factores Eucarióticos de Iniciación / Péptidos y Proteínas de Señalización Intracelular / Hipoxia Límite: Animals Idioma: En Revista: Life Sci Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipertrofia Ventricular Derecha / Remodelación Ventricular / Factores Eucarióticos de Iniciación / Péptidos y Proteínas de Señalización Intracelular / Hipoxia Límite: Animals Idioma: En Revista: Life Sci Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos