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The Conserved Phenylalanine in the Transmembrane Domain Enhances Heteromeric Interactions of Syndecans.
Kwon, Mi-Jung; Park, Jisu; Jang, Sinae; Eom, Chi-Yong; Oh, Eok-Soo.
Afiliación
  • Kwon MJ; From the Department of Life Sciences, Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea and.
  • Park J; From the Department of Life Sciences, Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea and.
  • Jang S; the Seoul Center, Korea Basic Science Institute, Seoul 136-075, Korea.
  • Eom CY; the Seoul Center, Korea Basic Science Institute, Seoul 136-075, Korea.
  • Oh ES; From the Department of Life Sciences, Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 120-750, Korea and OhES@ewha.ac.kr.
J Biol Chem ; 291(2): 872-81, 2016 Jan 08.
Article en En | MEDLINE | ID: mdl-26601939
The transmembrane domain (TMD) of the syndecans, a family of transmembrane heparin sulfate proteoglycans, is involved in forming homo- and heterodimers and oligomers that transmit signaling events. Recently, we reported that the unique phenylalanine in TMD positively regulates intramolecular interactions of syndecan-2. Besides the unique phenylalanine, syndecan-2 contains a conserved phenylalanine (SDC2-Phe-169) that is present in all syndecan TMDs, but its function has not been determined. We therefore investigated the structural role of SDC2-Phe-169 in syndecan TMDs. Replacement of SDC2-Phe-169 by tyrosine (S2F169Y) did not affect SDS-resistant homodimer formation but significantly reduced SDS-resistant heterodimer formation between syndecan-2 and -4, suggesting that SDC2-Phe-169 is involved in the heterodimerization/oligomerization of syndecans. Similarly, in an in vitro binding assay, a syndecan-2 mutant (S2(F169Y)) showed a significantly reduced interaction with syndecan-4. FRET assays showed that heteromolecular interactions between syndecan-2 and -4 were reduced in HEK293T cells transfected with S2(F169Y) compared with syndecan-2. Moreover, S2(F169Y) reduced downstream reactions mediated by the heterodimerization of syndecan-2 and -4, including Rac activity, cell migration, membrane localization of PKCα, and focal adhesion formation. The conserved phenylalanine in syndecan-1 and -3 also showed heterodimeric interaction with syndecan-2 and -4. Taken together, these findings suggest that the conserved phenylalanine in the TMD of syndecans is crucial in regulating heteromeric interactions of syndecans.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenilalanina / Secuencia Conservada / Sindecanos / Multimerización de Proteína Límite: Animals / Humans Idioma: En Revista: J Biol Chem Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenilalanina / Secuencia Conservada / Sindecanos / Multimerización de Proteína Límite: Animals / Humans Idioma: En Revista: J Biol Chem Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos