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Combined in utero hypoxia-ischemia and lipopolysaccharide administration in rats induces chorioamnionitis and a fetal inflammatory response syndrome.
Maxwell, Jessie R; Denson, Jesse L; Joste, Nancy E; Robinson, Shenandoah; Jantzie, Lauren L.
Afiliación
  • Maxwell JR; Departments of Pediatrics and Neurosciences, University of New Mexico, Albuquerque, NM, USA.
  • Denson JL; Departments of Pediatrics and Neurosciences, University of New Mexico, Albuquerque, NM, USA.
  • Joste NE; Department of Pathology, University of New Mexico, Albuquerque, NM, USA.
  • Robinson S; Departments of Neurosurgery and Neurology, Kirby Center for Neurobiology, Boston Children's Hospital, Harvard Medical School, Boston MA, USA.
  • Jantzie LL; Departments of Pediatrics and Neurosciences, University of New Mexico, Albuquerque, NM, USA. Electronic address: ljantzie@salud.unm.edu.
Placenta ; 36(12): 1378-84, 2015 Dec.
Article en En | MEDLINE | ID: mdl-26601766
INTRODUCTION: Preterm birth is a major cause of infant morbidity and long-term disability, and is associated with numerous central nervous system (CNS) deficits. Infants exposed to intrauterine inflammation, specifically chorioamnionitis, are at risk for very early preterm birth and neurological complications including cerebral palsy, epilepsy, and behavioral and cognitive deficits. However, placenta-brain axis abnormalities and their relationship to subsequent permanent CNS injury remain poorly defined. METHODS: Intrauterine injury was induced in rats on embryonic day 18 (E18) by transient systemic hypoxia-ischemia (TSHI) and intra-amniotic lipopolysaccharide (LPS) injection. Placenta, brain and serum were collected from E19 to postnatal day 0 (P0). Histology, TUNEL staining, western blot and multiplex immunoassays were used to quantify placental and brain abnormalities, and fetal serum cytokine levels. RESULTS: Prenatal TSHI + LPS caused acute and subacute placental injury hallmarked by inflammatory infiltrate, edema, hemorrhage and cell death along with placental increases in IL-1ß and TNFα. TSHI + LPS increased a diverse array of circulating inflammatory proteins including IL-1ß, TNFα, IL-6, IL-10, IL-4, IFNγ and CXCL1, both immediately after TSHI + LPS and in live born pups. CNS inflammation was characterized by increased CXCL1. DISCUSSION: Prenatal TSHI + LPS in rats induces placental injury and inflammation histologically consistent with chorioamnionitis, concomitant with elevated serum and CNS pro-inflammatory cytokines. This model accurately recapitulates key pathophysiological processes observed in extremely preterm infants including placental, fetal, and CNS inflammation. Further investigation into the mechanism of CNS injury following chorioamnionitis and the placental-brain axis will guide the use of future interventions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Encéfalo / Corioamnionitis / Hipoxia Fetal / Inflamación / Isquemia Tipo de estudio: Qualitative_research Límite: Animals / Pregnancy Idioma: En Revista: Placenta Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Encéfalo / Corioamnionitis / Hipoxia Fetal / Inflamación / Isquemia Tipo de estudio: Qualitative_research Límite: Animals / Pregnancy Idioma: En Revista: Placenta Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos