Corticotropin releasing hormone and Urocortin 2 activate inflammatory pathways in cultured trophoblast cell lines.

Novembri, Romina; De Clemente, Caterina; Funghi, Lucia; Torricelli, Michela; Voltolini, Chiara; Challis, John R; Petraglia, Felice

Novembri, Romina; De Clemente, Caterina; Funghi, Lucia; Torricelli, Michela; Voltolini, Chiara; Challis, John R; Petraglia, Felice.

Afiliación

Novembri R; Department of Molecular and Developmental Medicine, University of Siena Viale Bracci, Siena, Italy.
De Clemente C; Department of Biotechnology, University of Siena, via Fiorentina 1, 53100 Siena, Italy.
Funghi L; Department of Molecular and Developmental Medicine, University of Siena Viale Bracci, Siena, Italy.
Torricelli M; Department of Molecular and Developmental Medicine, University of Siena Viale Bracci, Siena, Italy.
Voltolini C; Department of Molecular and Developmental Medicine, University of Siena Viale Bracci, Siena, Italy.
Challis JR; The University of Western Australia M460A, 35 Stirling Highway, Crawley, WA 6009, Australia.
Petraglia F; Department of Molecular and Developmental Medicine, University of Siena Viale Bracci, Siena, Italy. Electronic address: felice.petraglia@unisi.it.

Eur J Obstet Gynecol Reprod Biol ; 195: 200-205, 2015 Dec.

Article en En | MEDLINE | ID: mdl-26588439

RESUMEN

OBJECTIVE: Embryo implantation and parturition are recognized as inflammatory events involving endocrine and immune system. NF-kB and MAPK are two transcription factor families involved in inflammation. A possible role of neuroendocrine mechanism in early pregnancy and delivery was proposed for the neuropeptides related to corticotropin releasing hormones (CRH), named Urocortins (Ucns). Experimental and clinical studies support a role for CRH, Ucn, Ucn2 and Ucn3 in the endocrine/immune modulation of inflammation in human trophoblast; however the intracellular mechanisms are not yet recognized. The aim of the present study was to evaluate which of these neuropeptides modulate NF-kB or MAPKs pathways. STUDY DESIGN: In Jeg-3 placental cell line the effect of CRH, Ucn, Ucn2 or Ucn3 on NF-kB and MAPKs pathways were evaluated using Western blot analysis. RESULTS: CRH induced the phosphorylation of MAPK subunits; Ucn2 was able to induce the phosphorylation of both NF-kB and MAPK subunits. Ucn and Ucn3 had no effects on these pathways. CONCLUSIONS: These data provide novel information on inflammatory process in trophoblast cells: Ucn2 is a potent pro-inflammatory neuropeptide via NF-kB and MAPK pathways and CRH via MAPK, and CRH and Ucn2 network participates in the inflammatory mechanisms of pregnancy and parturition.

Asunto(s)

Hormona Liberadora de Corticotropina/farmacología; Hormonas/farmacología; Proteínas Quinasas Activadas por Mitógenos/efectos de los fármacos; FN-kappa B/efectos de los fármacos; Trofoblastos/efectos de los fármacos; Urocortinas/farmacología; Western Blotting; Línea Celular Tumoral; Humanos; Proteína Quinasa 1 Activada por Mitógenos/efectos de los fármacos; Proteína Quinasa 1 Activada por Mitógenos/metabolismo; Proteína Quinasa 3 Activada por Mitógenos/efectos de los fármacos; Proteína Quinasa 3 Activada por Mitógenos/metabolismo; Proteínas Quinasas Activadas por Mitógenos/metabolismo; FN-kappa B/metabolismo; Subunidad p50 de NF-kappa B/efectos de los fármacos; Subunidad p50 de NF-kappa B/metabolismo; Transducción de Señal; Factor de Transcripción ReIA/efectos de los fármacos; Factor de Transcripción ReIA/metabolismo; Trofoblastos/metabolismo; Proteínas Quinasas p38 Activadas por Mitógenos/efectos de los fármacos; Proteínas Quinasas p38 Activadas por Mitógenos/metabolismo

Palabras clave

CRH; Jeg 3; MAPK; NF-kB; Urocortin 2

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trofoblastos / Hormona Liberadora de Corticotropina / FN-kappa B / Proteínas Quinasas Activadas por Mitógenos / Urocortinas / Hormonas Límite: Humans Idioma: En Revista: Eur J Obstet Gynecol Reprod Biol Año: 2015 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Irlanda

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