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Liberation of fermentable sugars from soybean hull biomass using ionic liquid 1-butyl-3-methylimidazolium acetate and their bioconversion to ethanol.
da Cunha-Pereira, Fernanda; Rech, Rosane; Záchia Ayub, Marco Antônio; Pinheiro Dillon, Aldo; Dupont, Jairton.
Afiliación
  • da Cunha-Pereira F; Department of Food Science & Technology, Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande Do Sul, Porto Alegre, RS ZC, 91501-970, Brazil.
  • Rech R; Department of Food Science & Technology, Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande Do Sul, Porto Alegre, RS ZC, 91501-970, Brazil.
  • Záchia Ayub MA; Department of Food Science & Technology, Biotechnology and Biochemical Engineering Laboratory (BiotecLab), Federal University of Rio Grande Do Sul, Porto Alegre, RS ZC, 91501-970, Brazil.
  • Pinheiro Dillon A; Biotechnology Institute, University of Caxias Do Sul, Brazil.
  • Dupont J; Laboratory of Molecular Catalysis, Federal University of Rio Grande Do Sul, Brazil.
Biotechnol Prog ; 32(2): 312-20, 2016 03.
Article en En | MEDLINE | ID: mdl-26588200
Optimized hydrolysis of lignocellulosic waste biomass is essential to achieve the liberation of sugars to be used in fermentation process. Ionic liquids (ILs), a new class of solvents, have been tested in the pretreatment of cellulosic materials to improve the subsequent enzymatic hydrolysis of the biomass. Optimized application of ILs on biomass is important to advance the use of this technology. In this research, we investigated the effects of using 1-butyl-3-methylimidazolium acetate ([bmim][Ac]) on the decomposition of soybean hull, an abundant cellulosic industrial waste. Reaction aspects of temperature, incubation time, IL concentration, and solid load were optimized before carrying out the enzymatic hydrolysis of this residue to liberate fermentable glucose. Optimal conditions were found to be 75°C, 165 min incubation time, 57% (mass fraction) of [bmim][Ac], and 12.5% solid loading. Pretreated soybean hull lost its crystallinity, which eased enzymatic hydrolysis, confirmed by Fourier Transform Infrared analysis. The enzymatic hydrolysis of the biomass using an enzyme complex from Penicillium echinulatum liberated 92% of glucose from the cellulose matrix. The hydrolysate was free of any toxic compounds, such as hydroxymethylfurfural and furfural. The obtained hydrolysate was tested for fermentation using Candida shehatae HM 52.2, which was able to convert glucose to ethanol at yields of 0.31. These results suggest the possible use of ILs for the pretreatment of some lignocellulosic waste materials, avoiding the formation of toxic compounds, to be used in second-generation ethanol production and other fermentation processes. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:312-320, 2016.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Celulosa / Etanol / Líquidos Iónicos / Fermentación / Imidazoles Idioma: En Revista: Biotechnol Prog Asunto de la revista: BIOTECNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glycine max / Celulosa / Etanol / Líquidos Iónicos / Fermentación / Imidazoles Idioma: En Revista: Biotechnol Prog Asunto de la revista: BIOTECNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos