Your browser doesn't support javascript.
loading
Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation.
Sharmin, Sazia; Taguchi, Atsuhiro; Kaku, Yusuke; Yoshimura, Yasuhiro; Ohmori, Tomoko; Sakuma, Tetsushi; Mukoyama, Masashi; Yamamoto, Takashi; Kurihara, Hidetake; Nishinakamura, Ryuichi.
Afiliación
  • Sharmin S; Department of Kidney Development, Institute of Molecular Embryology and Genetics, and.
  • Taguchi A; Department of Kidney Development, Institute of Molecular Embryology and Genetics, and.
  • Kaku Y; Department of Kidney Development, Institute of Molecular Embryology and Genetics, and.
  • Yoshimura Y; Department of Kidney Development, Institute of Molecular Embryology and Genetics, and Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan;
  • Ohmori T; Department of Kidney Development, Institute of Molecular Embryology and Genetics, and.
  • Sakuma T; Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan;
  • Mukoyama M; Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan;
  • Yamamoto T; Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan;
  • Kurihara H; Division of Anatomy, Juntendo University School of Medicine, Tokyo, Japan; and.
  • Nishinakamura R; Department of Kidney Development, Institute of Molecular Embryology and Genetics, and Japan Science and Technology Agency, CREST, Kumamoto, Japan ryuichi@kumamoto-u.ac.jp.
J Am Soc Nephrol ; 27(6): 1778-91, 2016 06.
Article en En | MEDLINE | ID: mdl-26586691
Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney development has been minimal because of limited access to embryonic kidneys. We previously reported the induction of three-dimensional primordial glomeruli from human induced pluripotent stem (iPS) cells. Here, using transcription activator-like effector nuclease-mediated homologous recombination, we generated human iPS cell lines that express green fluorescent protein (GFP) in the NPHS1 locus, which encodes nephrin, and we show that GFP expression facilitated accurate visualization of nephrin-positive podocyte formation in vitro These induced human podocytes exhibited apicobasal polarity, with nephrin proteins accumulated close to the basal domain, and possessed primary processes that were connected with slit diaphragm-like structures. Microarray analysis of sorted iPS cell-derived podocytes identified well conserved marker gene expression previously shown in mouse and human podocytes in vivo Furthermore, we developed a novel transplantation method using spacers that release the tension of host kidney capsules, thereby allowing the effective formation of glomeruli from human iPS cell-derived nephron progenitors. The human glomeruli were vascularized with the host mouse endothelial cells, and iPS cell-derived podocytes with numerous cell processes accumulated around the fenestrated endothelial cells. Therefore, the podocytes generated from iPS cells retain the podocyte-specific molecular and structural features, which will be useful for dissecting human glomerular development and diseases.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Podocitos / Células Madre Pluripotentes Inducidas / Glomérulos Renales Límite: Animals / Humans Idioma: En Revista: J Am Soc Nephrol Asunto de la revista: NEFROLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Podocitos / Células Madre Pluripotentes Inducidas / Glomérulos Renales Límite: Animals / Humans Idioma: En Revista: J Am Soc Nephrol Asunto de la revista: NEFROLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos