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2-Furaldehyde diethyl acetal from tender coconut water (Cocos nucifera) attenuates biofilm formation and quorum sensing-mediated virulence of Chromobacterium violaceum and Pseudomonas aeruginosa.
Sethupathy, Sivasamy; Nithya, Chari; Pandian, Shunmugiah Karutha.
Afiliación
  • Sethupathy S; a Department of Biotechnology , Alagappa University , Karaikudi , India.
  • Nithya C; a Department of Biotechnology , Alagappa University , Karaikudi , India.
  • Pandian SK; a Department of Biotechnology , Alagappa University , Karaikudi , India.
Biofouling ; 31(9-10): 721-33, 2015.
Article en En | MEDLINE | ID: mdl-26571230
The aim of this study was to evaluate the anti-biofilm and quorum sensing inhibitory (QSI) potential of tender coconut water (TCW) against Chromobacterium violaceum and Pseudomonas aeruginosa. TCW significantly inhibited the QS regulated violacein, virulence factors and biofilm production without affecting their growth. qRT-PCR analysis revealed the down-regulation of autoinducer synthase, transcriptional regulator and virulence genes. Mass-spectrometric analysis of a petroleum ether extract of the TCW hydrolyte revealed that 2-furaldehyde diethyl acetal (2FDA) and palmitic acid (PA) are the major compounds. In vitro bioassays confirmed the ability of 2FDA to inhibit the biofilm formation and virulence factors. In addition, the combination of PA with 2FDA resulted in potent inhibition of biofilm formation and virulence factors. The results obtained strongly suggest that TCW can be exploited as a base for designing a novel antipathogenic drug formulation to treat biofilm mediated infections caused by P. aeruginosa.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Chromobacterium / Cocos / Biopelículas / Percepción de Quorum / Furaldehído País/Región como asunto: Asia Idioma: En Revista: Biofouling Asunto de la revista: BIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Chromobacterium / Cocos / Biopelículas / Percepción de Quorum / Furaldehído País/Región como asunto: Asia Idioma: En Revista: Biofouling Asunto de la revista: BIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido