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Functional and expression analyses of transcripts based on full-length cDNAs of Sorghum bicolor.
Shimada, Setsuko; Makita, Yuko; Kuriyama-Kondou, Tomoko; Kawashima, Mika; Mochizuki, Yoshiki; Hirakawa, Hideki; Sato, Shusei; Toyoda, Tetsuro; Matsui, Minami.
Afiliación
  • Shimada S; Synthetic Genomics Research Group, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
  • Makita Y; Synthetic Genomics Research Group, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
  • Kuriyama-Kondou T; Synthetic Genomics Research Group, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
  • Kawashima M; Synthetic Genomics Research Group, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
  • Mochizuki Y; RIKEN Advanced Center for Computing and Communication (ACCC), Wako, Saitama 351-0198, Japan.
  • Hirakawa H; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Sato S; Kazusa DNA Research Institute, Kisarazu, Chiba 292-0818, Japan.
  • Toyoda T; RIKEN Advanced Center for Computing and Communication (ACCC), Wako, Saitama 351-0198, Japan.
  • Matsui M; Synthetic Genomics Research Group, Biomass Engineering Research Division, RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan minami@riken.jp.
DNA Res ; 22(6): 485-93, 2015 Dec.
Article en En | MEDLINE | ID: mdl-26546227
Sorghum bicolor is one of the most important crops for food and bioethanol production. Its small diploid genome and resistance to environmental stress make sorghum an attractive model for studying the functional genomics of the Saccharinae and other C4 grasses. We analyzed the domain-based functional annotation of the cDNAs using the gene ontology (GO) categories for molecular function to characterize all the genes cloned in the full-length cDNA library of sorghum. The sorghum cDNA library successfully captured a wide range of cDNA-encoded proteins with various functions. To characterize the protein function of newly identified cDNAs, a search of their deduced domains and comparative analyses in the Oryza sativa and Zea mays genomes were carried out. Furthermore, genes on the sense strand corresponding to antisense transcripts were classified based on the GO of molecular function. To add more information about these genes, we have analyzed the expression profiles using RNA-Seq of three tissues (spikelet, seed and stem) during the starch-filling phase. We performed functional analysis of tissue-specific genes and expression analysis of genes of starch biosynthesis enzymes. This functional analysis of sorghum full-length cDNAs and the transcriptome information will facilitate further analysis of the Saccharinae and grass families.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / ADN Complementario / Regulación de la Expresión Génica de las Plantas / Zea mays / Sorghum Idioma: En Revista: DNA Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / ADN Complementario / Regulación de la Expresión Génica de las Plantas / Zea mays / Sorghum Idioma: En Revista: DNA Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido