Two-phase and three-phase liquid-phase microextraction of hydrochlorothiazide and triamterene in urine samples.
Biomed Chromatogr
; 30(7): 1022-1028, 2016 Jul.
Article
en En
| MEDLINE
| ID: mdl-26542449
This paper reports the applicability of two-phase and three-phase hollow fiber based liquid-phase microextraction (HF-LPME) for the extraction of hydrochlorothiazide (HYD) and triamterene (TRM) from human urine. The HYD in two-phase HF-LPME is extracted from 24 mL of the aqueous sample into an organic phase with microliter volume located inside the pores and lumen of a polypropylene hollow fiber as acceptor phase, but the TRM in three-phase HF-LPME is extracted from aqueous donor phase to organic phase and then back-extracted to the aqueous acceptor phase, which can be directly injected into HPLC for analysis. Under optimized conditions preconcentration factors of HYD and TRM were obtained as 128 and 239, respectively. The calibration curves were linear (R(2) ≥ 0.995) in the concentration range of 1.0-100 µg/L for HYD and 2.0-100 µg/L for TRM. The limits of detection for HYD and TRM were 0.5 µg/L. The intra-day and inter-day RSD based on four replicates were obtained as ≤5.8 and ≤9.3%, respectively. The methods were successfully applied for determining the concentration of the drugs in urine samples. Copyright © 2015 John Wiley & Sons, Ltd.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Triantereno
/
Diuréticos
/
Microextracción en Fase Líquida
/
Hidroclorotiazida
Límite:
Humans
Idioma:
En
Revista:
Biomed Chromatogr
Año:
2016
Tipo del documento:
Article
País de afiliación:
Irán
Pais de publicación:
Reino Unido