Your browser doesn't support javascript.
loading
Renoprotective effects of berberine and its potential effect on the expression of ß-arrestins and intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in streptozocin-diabetic nephropathy rats.
Tang, Li-Qin; Ni, Wei-Jian; Cai, Ming; Ding, Hai-Hua; Liu, Sheng; Zhang, Shan-Tang.
Afiliación
  • Tang LQ; Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui Province, PR China.
  • Ni WJ; Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui Province, PR China.
  • Cai M; Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui Province, PR China.
  • Ding HH; Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui Province, PR China.
  • Liu S; Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui Province, PR China.
  • Zhang ST; Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei, Anhui Province, PR China.
J Diabetes ; 8(5): 693-700, 2016 Sep.
Article en En | MEDLINE | ID: mdl-26531813
BACKGROUND: Berberine has been shown to exert protective effects against diabetic nephropathy (DN), but the mechanisms involved have not been fully characterized. The aim of the present study was to explore the effects of berberine on the expression of ß-arrestins, intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in DN rat kidneys and investigate the underlying molecular mechanisms. METHODS: To create the DN model, rats fed a high-fat and high-glucose diet were injected with a single dose of streptozotocin (35 mg/kg, i.p.). Then, DN rats were either treated or not with berberine (50, 100, 200 mg/kg per day, i.g., 8 weeks). Periodic acid-Schiff staining was used to evaluate renal histopathological changes. Renal tissue levels of ß-arrestin 1 and ß-arrestin 2 were determined by Western blot analysis, whereas immunohistochemistry was used to determine renal ICAM-1 and VCAM-1 levels. RESULTS: Berberine (100, 200 mg/kg) ameliorated the histopathological changes in the diabetic kidney. Western blot analysis revealed significant increases in ICAM-1 and VCAM-1 levels in the kidneys of DN rats, which were reversed by treatment with 100 and 200 mg/kg berberine. In addition, berberine treatment (50, 100, 200 mg/kg) increased diabetic-induced decreases in ß-arrestin 1 and ß-arrestin 2. CONCLUSIONS: Berberine exhibited renoprotective effects in DN rats. The underlying molecular mechanisms may be associated with changes in the levels and regulation of ß-arrestin expression, as well as ICAM-1 and VCAM-1 levels in the rat kidney.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Berberina / Molécula 1 de Adhesión Intercelular / Molécula 1 de Adhesión Celular Vascular / Diabetes Mellitus Experimental / Nefropatías Diabéticas / Beta-Arrestinas Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: J Diabetes Asunto de la revista: ENDOCRINOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Australia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Berberina / Molécula 1 de Adhesión Intercelular / Molécula 1 de Adhesión Celular Vascular / Diabetes Mellitus Experimental / Nefropatías Diabéticas / Beta-Arrestinas Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: J Diabetes Asunto de la revista: ENDOCRINOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Australia