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The EF-Hand Ca2+ Binding Protein MICU Choreographs Mitochondrial Ca2+ Dynamics in Arabidopsis.
Wagner, Stephan; Behera, Smrutisanjita; De Bortoli, Sara; Logan, David C; Fuchs, Philippe; Carraretto, Luca; Teardo, Enrico; Cendron, Laura; Nietzel, Thomas; Füßl, Magdalena; Doccula, Fabrizio G; Navazio, Lorella; Fricker, Mark D; Van Aken, Olivier; Finkemeier, Iris; Meyer, Andreas J; Szabò, Ildikò; Costa, Alex; Schwarzländer, Markus.
Afiliación
  • Wagner S; Plant Energy Biology Lab, Institute of Crop Science and Resource Conservation, University of Bonn, 53113 Bonn, Germany.
  • Behera S; Department of Biosciences, University of Milan, 20133 Milan, Italy.
  • De Bortoli S; Department of Biology, University of Padova, 35121 Padova, Italy.
  • Logan DC; Université d'Angers, INRA, Agrocampus Ouest, UMR 1345 Institut de Recherche en Horticulture et Semences, F-49045 Angers, France.
  • Fuchs P; Plant Energy Biology Lab, Institute of Crop Science and Resource Conservation, University of Bonn, 53113 Bonn, Germany.
  • Carraretto L; Department of Biology, University of Padova, 35121 Padova, Italy.
  • Teardo E; Department of Biology, University of Padova, 35121 Padova, Italy.
  • Cendron L; Department of Biology, University of Padova, 35121 Padova, Italy.
  • Nietzel T; Plant Energy Biology Lab, Institute of Crop Science and Resource Conservation, University of Bonn, 53113 Bonn, Germany.
  • Füßl M; Plant Proteomics Group, Max-Planck-Institute for Plant Breeding Research, 50829 Cologne, Germany.
  • Doccula FG; Department of Biosciences, University of Milan, 20133 Milan, Italy.
  • Navazio L; Department of Biology, University of Padova, 35121 Padova, Italy.
  • Fricker MD; Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.
  • Van Aken O; ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA 6009, Australia.
  • Finkemeier I; Plant Proteomics Group, Max-Planck-Institute for Plant Breeding Research, 50829 Cologne, Germany Institute for Plant Biology and Biotechnology, University of Münster, 48149 Münster, Germany.
  • Meyer AJ; Department Chemical Signalling, Institute of Crop Science and Resource Conservation, University of Bonn, 53113 Bonn, Germany.
  • Szabò I; Department of Biology, University of Padova, 35121 Padova, Italy.
  • Costa A; Department of Biosciences, University of Milan, 20133 Milan, Italy Institute of Biophysics, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy.
  • Schwarzländer M; Plant Energy Biology Lab, Institute of Crop Science and Resource Conservation, University of Bonn, 53113 Bonn, Germany markus.schwarzlander@uni-bonn.de.
Plant Cell ; 27(11): 3190-212, 2015 Nov.
Article en En | MEDLINE | ID: mdl-26530087
Plant organelle function must constantly adjust to environmental conditions, which requires dynamic coordination. Ca(2+) signaling may play a central role in this process. Free Ca(2+) dynamics are tightly regulated and differ markedly between the cytosol, plastid stroma, and mitochondrial matrix. The mechanistic basis of compartment-specific Ca(2+) dynamics is poorly understood. Here, we studied the function of At-MICU, an EF-hand protein of Arabidopsis thaliana with homology to constituents of the mitochondrial Ca(2+) uniporter machinery in mammals. MICU binds Ca(2+) and localizes to the mitochondria in Arabidopsis. In vivo imaging of roots expressing a genetically encoded Ca(2+) sensor in the mitochondrial matrix revealed that lack of MICU increased resting concentrations of free Ca(2+) in the matrix. Furthermore, Ca(2+) elevations triggered by auxin and extracellular ATP occurred more rapidly and reached higher maximal concentrations in the mitochondria of micu mutants, whereas cytosolic Ca(2+) signatures remained unchanged. These findings support the idea that a conserved uniporter system, with composition and regulation distinct from the mammalian machinery, mediates mitochondrial Ca(2+) uptake in plants under in vivo conditions. They further suggest that MICU acts as a throttle that controls Ca(2+) uptake by moderating influx, thereby shaping Ca(2+) signatures in the matrix and preserving mitochondrial homeostasis. Our results open the door to genetic dissection of mitochondrial Ca(2+) signaling in plants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al Calcio / Arabidopsis / Motivos EF Hand / Proteínas de Arabidopsis / Mitocondrias Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al Calcio / Arabidopsis / Motivos EF Hand / Proteínas de Arabidopsis / Mitocondrias Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2015 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido