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CDP-choline modulates matrix metalloproteinases in rat sciatic injury.
Gundogdu, Elif Basaran; Bekar, Ahmet; Turkyilmaz, Mesut; Gumus, Abdullah; Kafa, Ilker Mustafa; Cansev, Mehmet.
Afiliación
  • Gundogdu EB; Department of Neurosurgery, Uludag University Medical School, Bursa, Turkey.
  • Bekar A; Department of Neurosurgery, Uludag University Medical School, Bursa, Turkey. Electronic address: abekar@uludag.edu.tr.
  • Turkyilmaz M; Department of Pharmacology, Uludag University Medical School, Bursa, Turkey.
  • Gumus A; Uludag University Medical School, Bursa, Turkey.
  • Kafa IM; Department of Anatomy, Uludag University Medical School, Bursa, Turkey.
  • Cansev M; Department of Pharmacology, Uludag University Medical School, Bursa, Turkey.
J Surg Res ; 200(2): 655-63, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26521098
BACKGROUND: CDP-choline (cytidine-5'-diphosphocholine) improves functional recovery, promotes nerve regeneration, and decreases perineural scarring in rat peripheral nerve injury. The aim of the present study was to investigate the mechanism of action of CDP-choline with regard to matrix metalloproteinase (MMP) activity in the rat-transected sciatic nerve injury model. MATERIALS AND METHODS: Male Wistar rats were randomized into Sham, Saline, and CDP-choline groups. Rats in Sham group received Sham surgery, whereas rats in Saline and CDP-choline groups underwent right sciatic nerve transection followed by immediate primary saturation and injected intraperitoneally with 0.9% NaCl (1 mL/kg) and CDP-choline (600 µg/kg), respectively. Sciatic nerve samples were obtained 1, 3, and 7 d after the surgery and analyzed for levels and activities of MMP-2 and MMP-9, levels of tissue inhibitor of metalloproteinases-1 (TIMP-1) and TIMP-3, and axonal regeneration. RESULTS: CDP-choline treatment decreased the levels and activities of MMP-2 and MMP-9, whereas increasing levels of TIMP-1 and TIMP-3 significantly on the third and seventh day after injury compared to Saline group. In addition, CDP-choline administration resulted in new axon formation and formation and advancement of myelination on newly formed islets (compartments) of axonal regrowth. CONCLUSIONS: Our data show, for the first time, that CDP-choline modulates MMP activity and promotes the expression of TIMPs to stimulate axonal regeneration. These data help to explain one mechanism by which CDP-choline provides neuroprotection in peripheral nerve injury.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Ciático / Fármacos Neuroprotectores / Metaloproteinasa 2 de la Matriz / Metaloproteinasa 9 de la Matriz / Citidina Difosfato Colina / Traumatismos de los Nervios Periféricos / Regeneración Nerviosa Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: J Surg Res Año: 2016 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Ciático / Fármacos Neuroprotectores / Metaloproteinasa 2 de la Matriz / Metaloproteinasa 9 de la Matriz / Citidina Difosfato Colina / Traumatismos de los Nervios Periféricos / Regeneración Nerviosa Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: J Surg Res Año: 2016 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos