Analysis of Three-Dimensional Structures of Exocyst Components.
Methods Mol Biol
; 1369: 191-204, 2016.
Article
en En
| MEDLINE
| ID: mdl-26519314
The exocyst is an octameric protein complex implicated in tethering secretory vesicles to the plasma membrane during exocytosis. To provide a mechanistic understanding of how it functions, it is of critical importance to elucidate its three-dimensional structure. This chapter briefly describes the protocols used in our structure determination of Exo70p and Exo84p, two subunits of the exocyst from Saccharomyces cerevisiae. Folding and domain arrangements of both proteins are predicted using bioinformatics tools. Limited proteolysis is carried out to define the boundaries of folded structures, which guides the design of suitable constructs for protein crystallization. The solved structures of both proteins validate the strategy and suggest it might be also used for structural studies of other proteins alike.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Conformación Proteica
/
Saccharomyces cerevisiae
/
Modelos Moleculares
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Proteínas de Saccharomyces cerevisiae
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Complejos Multiproteicos
/
Exocitosis
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article
País de afiliación:
Austria
Pais de publicación:
Estados Unidos