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Importance of Endogenous Atrial and Brain Natriuretic Peptides in Murine Embryonic Vascular and Organ Development.
Tokudome, Takeshi; Kishimoto, Ichiro; Shindo, Takayuki; Kawakami, Hayato; Koyama, Teruhide; Otani, Kentaro; Nishimura, Hirohito; Miyazato, Mikiya; Kohno, Masakazu; Nakao, Kazuwa; Kangawa, Kenji.
Afiliación
  • Tokudome T; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Kishimoto I; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Shindo T; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Kawakami H; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Koyama T; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Otani K; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Nishimura H; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Miyazato M; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Kohno M; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Nakao K; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
  • Kangawa K; Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan; Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan; Department of Anatomy (H.K.), Kyorin U
Endocrinology ; 157(1): 358-67, 2016 Jan.
Article en En | MEDLINE | ID: mdl-26517044
Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) bind to the receptor guanylyl cyclase (GC)-A, leading to diuresis, natriuresis, and blood vessel dilation. In addition, ANP and BNP have various angiogenic properties in ischemic tissue. When breeding mice devoid of GC-A, we noted significant skewing of the Mendelian ratio in the offspring, suggesting embryonic lethality due to knockout of GC-A. Consequently, we here investigated the roles of endogenous ANP and BNP in embryonic neovascularization and organ morphogenesis. Embryos resulting from GC-A(-/-) × GC-A(+/-) crosses developed hydrops fetalis (HF) beginning at embryonic day (E)14.5. All embryos with HF had the genotype GC-A(-/-). At E17.5, 33.3% (12 of 36) of GC-A(-/-) embryos had HF, and all GC-A(-/-) embryos with HF were dead. Beginning at E16.0, HF-GC-A(-/-) embryos demonstrated poorly developed superficial vascular vessels and sc hemorrhage, the fetal side of the placenta appeared ischemic, and vitelline vessels on the yolk sac were poorly developed. Furthermore, HF-GC-A(-/-) embryos also showed abnormal constriction of umbilical cord vascular vessels, few cardiac trabeculae and a thin compact zone, hepatic hemorrhage, and poor bone development. Electron microscopy of E16.5 HF-GC-A(-/-) embryos revealed severe vacuolar degeneration in endothelial cells, and the expected 3-layer structure of the smooth muscle wall of the umbilical artery was indistinct. These data demonstrate the importance of the endogenous ANP/BNP-GC-A system not only in the neovascularization of ischemic tissues but also in embryonic vascular development and organ morphogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor Natriurético Atrial / Receptores del Factor Natriurético Atrial / Regulación del Desarrollo de la Expresión Génica / Neovascularización Fisiológica / Péptido Natriurético Encefálico / Organogénesis / Embrión de Mamíferos Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Endocrinology Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor Natriurético Atrial / Receptores del Factor Natriurético Atrial / Regulación del Desarrollo de la Expresión Génica / Neovascularización Fisiológica / Péptido Natriurético Encefálico / Organogénesis / Embrión de Mamíferos Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Endocrinology Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos