Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures.
J Biosci Bioeng
; 121(3): 317-24, 2016 Mar.
Article
en En
| MEDLINE
| ID: mdl-26454770
A human hybrid cell line, F2N78, was developed by somatic fusion of HEK293 and Namalwa cells for the production recombinant biopharmaceutical proteins. In this study, we performed perfusion culture to verify its potential in culture process used for human cell expression platform. Cell viability could be maintained over 90% and high viable cell density was obtained at higher than 1.0 × 10(7) cells/mL by bleeding process in perfusion culture. The cells were adapted well in both culture modes, but there were apparent differences in protein quality. Compared to fed-batch culture, degalactosylated forms such as G0F and G0 as well as Man5 showed no significant increases in perfusion culture. In terms of charge variants, acidic peaks increased, whereas main peaks constantly decreased according to the length of culture period in both methods.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes
/
Técnicas de Cultivo de Célula
/
Técnicas de Cultivo Celular por Lotes
/
Células Híbridas
/
Anticuerpos
Límite:
Humans
Idioma:
En
Revista:
J Biosci Bioeng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Corea del Sur
Pais de publicación:
Japón