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Comparative Transcriptome Analysis Reveals Cool Virulence Factors of Ralstonia solanacearum Race 3 Biovar 2.
Meng, Fanhong; Babujee, Lavanya; Jacobs, Jonathan M; Allen, Caitilyn.
Afiliación
  • Meng F; Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin, 53706, United States of America.
  • Babujee L; Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin, 53706, United States of America.
  • Jacobs JM; Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin, 53706, United States of America.
  • Allen C; Department of Plant Pathology, University of Wisconsin-Madison, Madison, Wisconsin, 53706, United States of America.
PLoS One ; 10(10): e0139090, 2015.
Article en En | MEDLINE | ID: mdl-26445498
While most strains of the plant pathogenic bacterium Ralstonia solanacearum are tropical, the race 3 biovar 2 (R3bv2) subgroup attacks plants in cooler climates. To identify mechanisms underlying this trait, we compared the transcriptional profiles of R. solanacearum R3bv2 strain UW551 and tropical strain GMI1000 at 20°C and 28°C, both in culture and during tomato pathogenesis. 4.2% of the ORFs in the UW551 genome and 7.9% of the GMI1000 ORFs were differentially expressed by temperature in planta. The two strains had distinct transcriptional responses to temperature change. GMI1000 up-regulated several stress response genes at 20°C, apparently struggling to cope with plant defenses. At the cooler temperature, R3bv2 strain UW551 up-regulated a cluster encoding a mannose-fucose binding lectin, LecM; a quorum sensing-dependent protein, AidA; and a related hypothetical protein, AidC. The last two genes are absent from the GMI1000 genome. In UW551, all three genes were positively regulated by the adjacent SolI/R quorum sensing system. These temperature-responsive genes were required for full virulence in R3bv2. Mutants lacking lecM, aidA, or aidC were each significantly more reduced in virulence on tomato at 20°C than at 28°C in both a naturalistic soil soak inoculation assay and when they were inoculated directly into tomato stems. The lecM and aidC mutants also survived poorly in potato tubers at the seed tuber storage temperature of 4°C, and the lecM mutant was defective in biofilm formation in vitro. Together, these results suggest novel mechanisms, including a lectin, are involved in the unique temperate epidemiology of R3bv2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Solanum lycopersicum / Factores de Virulencia / Ralstonia solanacearum / Transcriptoma Tipo de estudio: Prognostic_studies Aspecto: Determinantes_sociais_saude Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Solanum lycopersicum / Factores de Virulencia / Ralstonia solanacearum / Transcriptoma Tipo de estudio: Prognostic_studies Aspecto: Determinantes_sociais_saude Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos