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Genome Editing in Rats Using TALE Nucleases.
Tesson, Laurent; Remy, Séverine; Ménoret, Séverine; Usal, Claire; Thinard, Reynald; Savignard, Chloé; De Cian, Anne; Giovannangeli, Carine; Concordet, Jean-Paul; Anegon, Ignacio.
Afiliación
  • Tesson L; Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France. laurent.tesson@univ-nantes.fr.
  • Remy S; ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France. laurent.tesson@univ-nantes.fr.
  • Ménoret S; INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France. laurent.tesson@univ-nantes.fr.
  • Usal C; Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.
  • Thinard R; ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.
  • Savignard C; INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
  • De Cian A; Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.
  • Giovannangeli C; ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.
  • Concordet JP; INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
  • Anegon I; Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.
Methods Mol Biol ; 1338: 245-59, 2016.
Article en En | MEDLINE | ID: mdl-26443226
The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Animales Modificados Genéticamente / Endonucleasas / Técnicas de Inactivación de Genes Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2016 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Animales Modificados Genéticamente / Endonucleasas / Técnicas de Inactivación de Genes Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2016 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos