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Direct injection human plasma analysis for the quantification of antihypertensive drugs for therapeutic drug monitoring using hydrophilic interaction liquid chromatography/electrospray ionization mass spectrometry.
Meimaroglou, Stefanos; Vonaparti, Ariadni; Migias, George; Gennimata, Dimitra; Poulou, Sofia; Panderi, Irene.
Afiliación
  • Meimaroglou S; University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Athens, Panepistimopolis-Zografou, 157 71 Athens, Greece.
  • Vonaparti A; University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Athens, Panepistimopolis-Zografou, 157 71 Athens, Greece.
  • Migias G; Athens General Hospital "Alexandra", 80 Vas. Sofias Avenue, 115 28 Athens, Greece.
  • Gennimata D; Athens General Hospital "Korgialenio-Benakio National Red Cross" Erithrou Stavrou 1, 11526 Athens, Greece.
  • Poulou S; Integrated Scientific Solutions, Protesilaou 101, 131 22 Athens, Greece.
  • Panderi I; University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Athens, Panepistimopolis-Zografou, 157 71 Athens, Greece. Electronic address: ipanderi@pharm.uoa.gr.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1004: 1-9, 2015 Nov 01.
Article en En | MEDLINE | ID: mdl-26432361
The concept of personalized medicine is related to the development of new sensitive, precise and accurate analytical methods for therapeutic drug monitoring. In this article a rapid, sensitive and specific method was developed for the quantification of aliskiren, losartan, valsartan and hydrochlorothiazide in human plasma. Sample preparation was performed by protein precipitation with acetonitrile followed by filtration. All analytes and the internal standard (tiamulin) were separated by hydrophilic interaction liquid chromatography using an X-Bridge-HILIC analytical column (150.0×2.1mm i.d., particle size 3.5µm) under isocratic elution. The mobile phase was composed of a 10% 5mM ammonium formate water solution pH 4.5, adjusted with formic acid, in acetonitrile and pumped at a flow rate of 0.25mLmin(-1). The assay was linear over the concentration range of 5-500ngmL(-1) for all the analytes. Intermediate precision was less than 5.2% over the tested concentration ranges. The method is the first reported application of HILIC in the analysis antihypertensives in human plasma. With a small sample size (50µL human plasma) and a run time less than 6.0min for each sample the method can be used to support a wide range of clinical studies and therapeutic drug monitoring.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía Liquida / Monitoreo de Drogas / Espectrometría de Masa por Ionización de Electrospray / Antihipertensivos Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2015 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía Liquida / Monitoreo de Drogas / Espectrometría de Masa por Ionización de Electrospray / Antihipertensivos Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2015 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Países Bajos