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Generation of a recombinant West Nile virus stably expressing the Gaussia luciferase for neutralization assay.
Zhang, Pan-Tao; Shan, Chao; Li, Xiao-Dan; Liu, Si-Qing; Deng, Cheng-Lin; Ye, Han-Qing; Shang, Bao-Di; Shi, Pei-Yong; Lv, Ming; Shen, Bei-Fen; Qin, Cheng-Feng; Zhang, Bo.
Afiliación
  • Zhang PT; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Shan C; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China.
  • Li XD; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Liu SQ; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China.
  • Deng CL; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China.
  • Ye HQ; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China.
  • Shang BD; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Shi PY; Novartis Institute for Tropical Diseases, Singapore.
  • Lv M; Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
  • Shen BF; Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
  • Qin CF; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
  • Zhang B; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China. Electronic address: zhangbo@wh.iov.cn.
Virus Res ; 211: 17-24, 2016 Jan 04.
Article en En | MEDLINE | ID: mdl-26415755
West Nile virus (WNV) is a neurotropic human pathogen that has caused increasing infected cases over recent years. There is currently no licensed vaccine or effective drug for prevention and treatment of WNV infection in humans. To facilitate antiviral drug discovery and neutralizing antibody detection, a WNV cDNA clone containing a luciferase reporter gene was constructed through incorporating Gaussia luciferase (Gluc) gene within the capsid-coding region of WNV genome. Transfection of BHK-21 cells with the cDNA clone-derived RNA generated luciferase reporter WNV (WNV-Gluc) and the stable WNV-Gluc with high titers (>10(7)PFU/ml) was obtained through plaque purification. Luciferase activity was used to effectively quantify the viral production of WNV-Gluc. Using the reporter virus WNV-Gluc, we developed a luciferase based assay in a 12-well format for evaluating neutralizing antibodies. The reporter virus could be a powerful tool for epidemiological investigation of WNV, vaccine evaluation, antiviral drug screening, and the study of WNV replication and pathogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus del Nilo Occidental / Pruebas de Neutralización / Anticuerpos Neutralizantes / Luciferasas Tipo de estudio: Evaluation_studies Límite: Animals / Humans Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus del Nilo Occidental / Pruebas de Neutralización / Anticuerpos Neutralizantes / Luciferasas Tipo de estudio: Evaluation_studies Límite: Animals / Humans Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos