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Low-dose ionizing radiation induces mitochondrial fusion and increases expression of mitochondrial complexes I and III in hippocampal neurons.
Chien, Ling; Chen, Wun-Ke; Liu, Szu-Ting; Chang, Chuang-Rung; Kao, Mou-Chieh; Chen, Kuan-Wei; Chiu, Shih-Che; Hsu, Ming-Ling; Hsiang, I-Chou; Chen, Yu-Jen; Chen, Linyi.
Afiliación
  • Chien L; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
  • Chen WK; Department of Radiation Oncology, MacKay Memorial Hospital, Taipei, Taiwan, R.O.C.
  • Liu ST; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
  • Chang CR; Department of Medical Science, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
  • Kao MC; Center for Brain Research, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
  • Chen KW; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
  • Chiu SC; Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, R.O.C.
  • Hsu ML; Department of Radiation Oncology, MacKay Memorial Hospital, Taipei, Taiwan, R.O.C.
  • Hsiang IC; Department of Radiation Oncology, MacKay Memorial Hospital, Taipei, Taiwan, R.O.C.
  • Chen YJ; Department of Radiation Oncology, MacKay Memorial Hospital, Taipei, Taiwan, R.O.C.
  • Chen L; Department of Radiation Oncology, MacKay Memorial Hospital, Taipei, Taiwan, R.O.C.
Oncotarget ; 6(31): 30628-39, 2015 Oct 13.
Article en En | MEDLINE | ID: mdl-26415228
High energy ionizing radiation can cause DNA damage and cell death. During clinical radiation therapy, the radiation dose could range from 15 to 60 Gy depending on targets. While 2 Gy radiation has been shown to cause cancer cell death, studies also suggest a protective potential by low dose radiation. In this study, we examined the effect of 0.2-2 Gy radiation on hippocampal neurons. Low dose 0.2 Gy radiation treatment increased the levels of MTT. Since hippocampal neurons are post-mitotic, this result reveals a possibility that 0.2 Gy irradiation may increase mitochondrial activity to cope with stimuli. Maintaining neural plasticity is an energy-demanding process that requires high efficient mitochondrial function. We thus hypothesized that low dose radiation may regulate mitochondrial dynamics and function to ensure survival of neurons. Our results showed that five days after 0.2 Gy irradiation, no obvious changes on neuronal survival, neuronal synapses, membrane potential of mitochondria, reactive oxygen species levels, and mitochondrial DNA copy numbers. Interestingly, 0.2 Gy irradiation promoted the mitochondria fusion, resulting in part from the increased level of a mitochondrial fusion protein, Mfn2, and inhibition of Drp1 fission protein trafficking to the mitochondria. Accompanying with the increased mitochondrial fusion, the expressions of complexes I and III of the electron transport chain were also increased. These findings suggest that, hippocampal neurons undergo increased mitochondrial fusion to modulate cellular activity as an adaptive mechanism in response to low dose radiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radiación Ionizante / Complejo III de Transporte de Electrones / Complejo I de Transporte de Electrón / Dinámicas Mitocondriales / Hipocampo / Mitocondrias / Neuronas Límite: Animals Idioma: En Revista: Oncotarget Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Radiación Ionizante / Complejo III de Transporte de Electrones / Complejo I de Transporte de Electrón / Dinámicas Mitocondriales / Hipocampo / Mitocondrias / Neuronas Límite: Animals Idioma: En Revista: Oncotarget Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos