Your browser doesn't support javascript.
loading
Protective effects of epoxypukalide on pancreatic ß-cells and glucose metabolism in STZ-induced diabetic mice.
López-Acosta, Jose F; Villa-Pérez, Pablo; Fernández-Díaz, Cristina M; Román, Daniel de Luis; Díaz-Marrero, Ana R; Cueto, Mercedes; Perdomo, Germán; Cózar-Castellano, Irene.
Afiliación
  • López-Acosta JF; a Instituto de Genética y Biología Molecular (University of Valladolid-CSIC) ; Valladolid , Spain.
  • Villa-Pérez P; f These authors contributed equally to this work.
  • Fernández-Díaz CM; a Instituto de Genética y Biología Molecular (University of Valladolid-CSIC) ; Valladolid , Spain.
  • Román Dde L; f These authors contributed equally to this work.
  • Díaz-Marrero AR; a Instituto de Genética y Biología Molecular (University of Valladolid-CSIC) ; Valladolid , Spain.
  • Cueto M; b Svo Endocrinología y Nutrición HCUVA; Centro de Investigación de Endocrinología y Nutrición (University of Valladolid); Valladolid , Spain.
  • Perdomo G; c Departamento de Química Orgánica ; Instituto Universitario de Bioorgánica "Antonio González" -CIBICAN; University of La Laguna; San Cristóbal de La Laguna , Spain.
  • Cózar-Castellano I; d Instituto de Productos Naturales y Agrobiología (CSIC) ; La Laguna , Tenerife , Spain.
Islets ; 7(2): e1078053, 2015.
Article en En | MEDLINE | ID: mdl-26406478
Diabetes is a consequence of a decrease on functional ß-cell mass. We have recently demonstrated that epoxypukalide (Epoxy) is a natural compound with beneficial effects on primary cultures of rat islets. In this study, we extend our previous investigations to test the hypothesis that Epoxy protects ß-cells and improves glucose metabolism in STZ-induced diabetic mice. We used 3-months old male mice that were treated with Epoxy at 200 µg/kg body weight. Glucose intolerance was induced by multiple intraperitoneal low-doses of streptozotocin (STZ) on 5 consecutive days. Glucose homeostasis was evaluated measuring plasma insulin levels and glucose tolerance. Histomorphometry was used to quantify the number of pancreatic ß-cells per islet. ß-cell proliferation was assessed by BrdU incorporation, and apoptosis by TUNEL staining. Epoxy treatment significantly improved glucose tolerance and plasma insulin levels. These metabolic changes were associated with increased ß-cell numbers, as a result of a two-fold increase in ß-cell proliferation and a 50% decrease in ß-cell death. Our results demonstrate that Epoxy improves whole-body glucose homeostasis by preventing pancreatic ß-cell death due to STZ-induced toxicity in STZ-treated mice.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diabetes Mellitus Experimental / Células Secretoras de Insulina / Glucosa / Hipoglucemiantes / Lactonas Límite: Animals Idioma: En Revista: Islets Asunto de la revista: ENDOCRINOLOGIA / GASTROENTEROLOGIA Año: 2015 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diabetes Mellitus Experimental / Células Secretoras de Insulina / Glucosa / Hipoglucemiantes / Lactonas Límite: Animals Idioma: En Revista: Islets Asunto de la revista: ENDOCRINOLOGIA / GASTROENTEROLOGIA Año: 2015 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos