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Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater.
Cornejová, Tatiana; Venglovsky, Jan; Gregova, Gabriela; Kmetova, Marta; Kmet, Vladimir.
Afiliación
  • Cornejová T; University of Veterinary Medicine and Pharmacy, Kosice, Slovakia.
  • Venglovsky J; University of Veterinary Medicine and Pharmacy, Kosice, Slovakia.
  • Gregova G; University of Veterinary Medicine and Pharmacy, Kosice, Slovakia.
  • Kmetova M; Faculty of Medicine, Department of Medical Microbiology and Clinical Microbiology, P. J. Safarik University, Kosice, Slovakia.
  • Kmet V; Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Ann Agric Environ Med ; 22(3): 447-50, 2015.
Article en En | MEDLINE | ID: mdl-26403112
INTRODUCTION AND OBJECTIVE: Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental E. coli and on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing E. coli isolated from an urban wastewater treatment plant. MATERIALS AND METHOD: Wastewater samples and artificially added polystyrene pellets were used as the source for E. coli isolation. Minimal inhibitory concentrations of 19 antibiotics were determined according to CLSI (2013). Biofilm formation was investigated by crystal violet or resazurin methods. CTX-M, carbapenemases, qnrS, mobile elements and virulence factors were determined by PCR. Clonal relatedness of strains was detected by principal component analysis by a Maldi biotyper. RESULTS: ESBL phenotype was detected in 26% of environmental strains. CTX-M, CMY-2 and qnrS genes of antibiotic resistance were detected. IMP gene together with integron 1 in one ertapenem resistant E. coli was also recorded. There was no evident correlation between antibiotic resistance, virulence and biofilm production. CONCLUSIONS: The results showed that the wastewater is a source of ESBLs, carbapenemases and plasmid fluoroquinolone resistance. Strains with biofilm production, antibiotic resistance of CTX-M group, CMY-2, qnrS genes and virulence factors present a potential environmental health risk.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopelículas / Genes MDR / Farmacorresistencia Bacteriana Múltiple / Escherichia coli / Antibacterianos País/Región como asunto: Europa Idioma: En Revista: Ann Agric Environ Med Asunto de la revista: SAUDE AMBIENTAL Año: 2015 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Polonia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopelículas / Genes MDR / Farmacorresistencia Bacteriana Múltiple / Escherichia coli / Antibacterianos País/Región como asunto: Europa Idioma: En Revista: Ann Agric Environ Med Asunto de la revista: SAUDE AMBIENTAL Año: 2015 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Polonia