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HAp granules encapsulated oxidized alginate-gelatin-biphasic calcium phosphate hydrogel for bone regeneration.
Sarker, Avik; Amirian, Jhaleh; Min, Young Ki; Lee, Byong Taek.
Afiliación
  • Sarker A; Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea.
  • Amirian J; Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea.
  • Min YK; Department of Physiology, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea; Institute of Tissue Regeneration, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea.
  • Lee BT; Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea; Institute of Tissue Regeneration, Soonchunhyang University, 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic
Int J Biol Macromol ; 81: 898-911, 2015 Nov.
Article en En | MEDLINE | ID: mdl-26394381
Bone repair in the critical size defect zone using 3D hydrogel scaffold is still a challenge in tissue engineering field. A novel type of hydrogel scaffold combining ceramic and polymer materials, therefore, was fabricated to meet this challenge. In this study, oxidized alginate-gelatin-biphasic calcium phosphate (OxAlg-Gel-BCP) and spherical hydroxyapatite (HAp) granules encapsulated OxAlg-Gel-BCP hydrogel complex were fabricated using freeze-drying method. Detailed morphological and material characterizations of OxAlg-Gel-BCP hydrogel (OGB00), 25wt% and 35wt% granules encapsulated hydrogel (OGB25 and OGB35) were carried out for micro-structure, porosity, chemical constituents, and compressive stress analysis. Cell viability, cell attachment, proliferation and differentiation behavior of rat bone marrow-derived stem cell (BMSC) on OGB00, OGB25 and OGB35 scaffolds were confirmed by MTT assay, Live-Dead assay, and confocal imaging in vitro experiments. Finally, OGB00 and OGB25 hydrogel scaffolds were implanted in the critical size defect of rabbit femoral chondyle for 4 and 8 weeks. The micro-CT analysis and histological studies conducted by H&E and Masson's trichrome demonstrated that a significantly higher (***p<0.001) and earlier bone formation happened in case of 25% HAp granules encapsulated OxAlg-Gel-BCP hydrogel than in OxAlg-Gel-BCP complex alone. All results taken together, HAp granules encapsulated OxAlg-Gel-BCP system can be a promising 3D hydrogel scaffold for the healing of a critical bone defect.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Fosfatos de Calcio / Durapatita / Hidrogel de Polietilenoglicol-Dimetacrilato / Alginatos / Gelatina Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Fosfatos de Calcio / Durapatita / Hidrogel de Polietilenoglicol-Dimetacrilato / Alginatos / Gelatina Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos