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Leucoreduction helps to preserve activity of antioxidant barrier enzymes in stored red blood cell concentrates.
Nedzi, M; Chabowska, A M; Rogowska, A; Boczkowska-Radziwon, B; Nedzi, A; Radziwon, P.
Afiliación
  • Nedzi M; Department of Hematology, Medical University of Bialystok, Bialystok, Poland.
  • Chabowska AM; Regional Centre for Transfusion Medicine in Bialystok, Bialystok, Poland.
  • Rogowska A; Regional Centre for Transfusion Medicine in Bialystok, Bialystok, Poland.
  • Boczkowska-Radziwon B; Regional Centre for Transfusion Medicine in Bialystok, Bialystok, Poland.
  • Nedzi A; Department of Pediatric Neurology, Medical University of Bialystok, Bialystok, Poland.
  • Radziwon P; Department of Hematology, Medical University of Bialystok, Bialystok, Poland.
Vox Sang ; 110(2): 126-33, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26389703
BACKGROUND: Oxidoreductive imbalance is a major cause of excessive haemolysis in in vitro conditions. Leucocytes and blood platelets present in red blood cell concentrates (RBCs) are one of the sources of free radicals, which have a significant effect on the status of stored erythrocytes. The study objective was to assess the effect of leucoreduction on the intensity of lipid peroxidation and the activity of antioxidant barrier enzymes in RBC. STUDY DESIGN AND METHODS: Red blood cell concentrates units obtained from 10 whole-blood units were split into two equal units, one of which was leucoreduced on the day of donation. Both units were stored for 35 days. The following markers of oxidoreductive balance were measured on day 0 (donation day) and on storage days 7, 14, 21 and 35: concentration of malondialdehyde (MDA) and activities of antioxidant barrier components, that is superoxide dismutase, glutathione peroxidase and glutathione reductase. RESULTS: Lipid peroxidation in leucodepleted units (LRBC) was slower than that in non-leucodepleted ones. The analysis of LRBC revealed statistically significant decrease in concentrations of MDA. The activities of superoxide dismutase, glutathione peroxidase and glutathione reductase were higher throughout the storage period as compared to non-leucoreduced RBC. Statistically significant differences between RBC and LRBC units were noted throughout the storage in the activity of lactate dehydrogenase, and concentrations of K(+) ions and free haemoglobin. CONCLUSIONS: Leucoreduction of RBC before storage helps to preserve the activity of antioxidant barrier enzymes in stored RBCs and significantly improves the quality of stored red blood cell components.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Superóxido Dismutasa / Conservación de la Sangre / Eritrocitos / Glutatión Peroxidasa Límite: Humans Idioma: En Revista: Vox Sang Año: 2016 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Superóxido Dismutasa / Conservación de la Sangre / Eritrocitos / Glutatión Peroxidasa Límite: Humans Idioma: En Revista: Vox Sang Año: 2016 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido