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Lack of Association between Polymorphisms of the TLR4 Gene and Infection with the Hepatitis B and C Viruses.
Pires-Neto, Orlando de Souza; de Sá, Keyla Santos Guedes; Santana, Barbara Brasil; Gomes, Samara Tatielle Monteiro; Amoras, Ednelza da Silva Graça; Conde, Simone Regina da Silva; Demachki, Sâmia; Azevedo, Vânia Nakauth; Martins-Feitosa, Rosimar Neris; Ishak, Marluísa de Oliveira Guimarães; Ishak, Ricardo; Vallinoto, Antonio Carlos Rosário.
Afiliación
  • Pires-Neto Ode S; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • de Sá KS; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Santana BB; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Gomes ST; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Amoras Eda S; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Conde SR; Faculdade de Medicina, Instituto de Ciências da Saúde, Universidade Federal do Pará, Umarizal, 66050-060 Belém, PA, Brazil.
  • Demachki S; Faculdade de Medicina, Instituto de Ciências da Saúde, Universidade Federal do Pará, Umarizal, 66050-060 Belém, PA, Brazil.
  • Azevedo VN; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Martins-Feitosa RN; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Ishak Mde O; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Ishak R; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
  • Vallinoto AC; Laboratorio de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, Brazil.
Mediators Inflamm ; 2015: 150673, 2015.
Article en En | MEDLINE | ID: mdl-26347404
Toll-like receptor 4 (TLR4) plays a crucial role in the early recognition of pathogenic microorganisms and provides an ideal model to investigate the consequences of genetic variation and susceptibility to diseases. The present study investigated the occurrence of the single nucleotide polymorphisms (SNPs) rs4986790 (A>G) and rs4986791 (C>T) in the TLR4 gene in chronic carriers of the hepatitis B (HBV) and C (HCV) viruses. A total of 420 blood samples were collected (HBV, 49; HCV, 72; and controls, 299) at the liver disease outpatient clinic of Hospital da Fundação Santa Casa de Misericórdia do Pará (FSCMPA). Genomic DNA extracted from leukocytes was subjected to real-time polymerase chain reaction (qPCR) analysis to identify the genetic profile of the participants. No significant differences were found in the allele and genotype frequencies between the infected participants and controls. No significant associations were found between the investigated polymorphisms and inflammatory activity, fibrosis, and the presence of cirrhosis; the same results were obtained in the haplotype analysis. The results showed a lack of association between the rs4986790 and rs4986791 SNPs and susceptibility to infection with HBV and HCV, as well as clinical and laboratory information of the patients.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepatitis C / Polimorfismo de Nucleótido Simple / Receptor Toll-Like 4 / Hepatitis B Tipo de estudio: Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Mediators Inflamm Asunto de la revista: BIOQUIMICA / PATOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepatitis C / Polimorfismo de Nucleótido Simple / Receptor Toll-Like 4 / Hepatitis B Tipo de estudio: Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Mediators Inflamm Asunto de la revista: BIOQUIMICA / PATOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos