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The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.
Kuroda, Kazunari; Kabata, Tamon; Hayashi, Katsuhiro; Maeda, Toru; Kajino, Yoshitomo; Iwai, Shintaro; Fujita, Kenji; Hasegawa, Kazuhiro; Inoue, Daisuke; Sugimoto, Naotoshi; Tsuchiya, Hiroyuki.
Afiliación
  • Kuroda K; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kurokazu0108@yahoo.co.jp.
  • Kabata T; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. tamonkabata@yahoo.co.jp.
  • Hayashi K; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. hayashikatsu830@aol.com.
  • Maeda T; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. torumaed@gmail.com.
  • Kajino Y; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. torumaed@gmail.com.
  • Iwai S; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. shntr.iwai@gmail.com.
  • Fujita K; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kkkkkenji76@yahoo.co.jp.
  • Hasegawa K; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. kazuhiro63hasegawa@gmail.com.
  • Inoue D; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. daisuke_i_0909@yahoo.co.jp.
  • Sugimoto N; Department of Physiology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan. ns@med.kanazawa-u.ac.jp.
  • Tsuchiya H; Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1, Takara-machi, Kanazawa, 920-8641, Japan. tsuchi@med.kanazawa-u.ac.jp.
BMC Musculoskelet Disord ; 16: 236, 2015 Sep 03.
Article en En | MEDLINE | ID: mdl-26336958
BACKGROUND: This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system. METHODS: ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes. RESULTS: Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups. CONCLUSIONS: Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adipocitos / Progresión de la Enfermedad / Comunicación Paracrina / Osteoartritis de la Rodilla / Trasplante de Células Madre Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: BMC Musculoskelet Disord Asunto de la revista: FISIOLOGIA / ORTOPEDIA Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adipocitos / Progresión de la Enfermedad / Comunicación Paracrina / Osteoartritis de la Rodilla / Trasplante de Células Madre Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: BMC Musculoskelet Disord Asunto de la revista: FISIOLOGIA / ORTOPEDIA Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido