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Heterogeneous Ribonucleoprotein K (hnRNP K) Binds miR-122, a Mature Liver-Specific MicroRNA Required for Hepatitis C Virus Replication.
Fan, Baochang; Sutandy, F X Reymond; Syu, Guan-Da; Middleton, Stefani; Yi, Guanghui; Lu, Kuan-Yi; Chen, Chien-Sheng; Kao, C Cheng.
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  • Fan B; From the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA;
  • Sutandy FX; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
  • Syu GD; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
  • Middleton S; From the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA;
  • Yi G; From the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA;
  • Lu KY; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
  • Chen CS; §Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan ckao@indiana.edu cchen103@gmail.com.
  • Kao CC; From the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA; ckao@indiana.edu cchen103@gmail.com.
Mol Cell Proteomics ; 14(11): 2878-86, 2015 Nov.
Article en En | MEDLINE | ID: mdl-26330540
Heterogeneous ribonucleoprotein K (hnRNP K) binds to the 5' untranslated region of the hepatitis C virus (HCV) and is required for HCV RNA replication. The hnRNP K binding site on HCV RNA overlaps with the sequence recognized by the liver-specific microRNA, miR-122. A proteome chip containing ∼17,000 unique human proteins probed with miR-122 identified hnRNP K as one of the strong binding proteins. In vitro kinetic study showed hnRNP K binds miR-122 with a nanomolar dissociation constant, in which the short pyrimidine-rich residues in the central and 3' portion of the miR-122 were required for hnRNP K binding. In liver hepatocytes, miR-122 formed a coprecipitable complex with hnRNP K. High throughput Illumina DNA sequencing of the RNAs precipitated with hnRNP K was enriched for mature miR-122. SiRNA knockdown of hnRNP K in human hepatocytes reduced the levels of miR-122. These results show that hnRNP K is a cellular protein that binds and affects the accumulation of miR-122. Its ability to also bind HCV RNA near the miR-122 binding site suggests a role for miR-122 recognition of HCV RNA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepacivirus / Hepatocitos / Ribonucleoproteína Heterogénea-Nuclear Grupo K / MicroARNs / Hígado Límite: Humans Idioma: En Revista: Mol Cell Proteomics Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hepacivirus / Hepatocitos / Ribonucleoproteína Heterogénea-Nuclear Grupo K / MicroARNs / Hígado Límite: Humans Idioma: En Revista: Mol Cell Proteomics Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos