Your browser doesn't support javascript.
loading
Lipidomic evidence that lowering the typical dietary palmitate to oleate ratio in humans decreases the leukocyte production of proinflammatory cytokines and muscle expression of redox-sensitive genes.
Kien, C Lawrence; Bunn, Janice Y; Fukagawa, Naomi K; Anathy, Vikas; Matthews, Dwight E; Crain, Karen I; Ebenstein, David B; Tarleton, Emily K; Pratley, Richard E; Poynter, Matthew E.
Afiliación
  • Kien CL; Department of Pediatrics, University of Vermont, Burlington, VT; Department of Medicine, University of Vermont, Burlington, VT. Electronic address: cl.kien@uvm.edu.
  • Bunn JY; Department of Medical Biostatistics, University of Vermont, Burlington, VT.
  • Fukagawa NK; Department of Medicine, University of Vermont, Burlington, VT.
  • Anathy V; Department of Pathology and Laboratory Medicine, University of Vermont, Burlington, VT.
  • Matthews DE; Department of Medicine, University of Vermont, Burlington, VT; Department of Chemistry, University of Vermont, Burlington, VT.
  • Crain KI; Department of Medicine, University of Vermont, Burlington, VT.
  • Ebenstein DB; Department of Medicine, University of Vermont, Burlington, VT.
  • Tarleton EK; College of Medicine Clinical Research Center, University of Vermont, Burlington, VT.
  • Pratley RE; Translational Research Institute for Metabolism and Diabetes, Sanford-Burnham Medical Research Institute, Orlando, FL.
  • Poynter ME; Department of Medicine, University of Vermont, Burlington, VT.
J Nutr Biochem ; 26(12): 1599-606, 2015 Dec.
Article en En | MEDLINE | ID: mdl-26324406
We recently reported that lowering the high, habitual palmitic acid (PA) intake in ovulating women improved insulin sensitivity and both inflammatory and oxidative stress. In vitro studies indicate that PA can activate both cell membrane toll-like receptor-4 and the intracellular nucleotide oligomerization domain-like receptor protein (NLRP3). To gain further insight into the relevance to human metabolic disease of dietary PA, we studied healthy, lean and obese adults enrolled in a randomized, crossover trial comparing 3-week, high-PA (HPA) and low-PA/high-oleic-acid (HOA) diets. After each diet, both hepatic and peripheral insulin sensitivities were measured, and we assessed cytokine concentrations in plasma and in supernatants derived from lipopolysaccharide-stimulated peripheral blood mononuclear cells (PBMCs) as well as proinflammatory gene expression in skeletal muscle. Insulin sensitivity was unaffected by diet. Plasma concentration of tumor necrosis factor-α was higher during the HPA diet. Lowering the habitually high PA intake by feeding the HOA diet resulted in lower secretion of interleukin (IL)-1ß, IL-18, IL-10, and tumor necrosis factor-α by PBMCs, as well as lower relative mRNA expression of cJun and NLRP3 in muscle. Principal components analysis of 156 total variables coupled to analysis of covariance indicated that the mechanistic pathway for the differential dietary effects on PBMCs involved changes in the PA/OA ratio of tissue lipids. Our results indicate that lowering the dietary and tissue lipid PA/OA ratio resulted in lower leukocyte production of proinflammatory cytokines and muscle expression of redox-sensitive genes, but the relevance to diabetes risk is uncertain.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Músculo Esquelético / Ácido Palmítico / Ácido Oléico / Dieta / Leucocitos / Lípidos Tipo de estudio: Clinical_trials / Diagnostic_studies Límite: Adolescent / Adult / Female / Humans / Male Idioma: En Revista: J Nutr Biochem Asunto de la revista: BIOQUIMICA / CIENCIAS DA NUTRICAO Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Músculo Esquelético / Ácido Palmítico / Ácido Oléico / Dieta / Leucocitos / Lípidos Tipo de estudio: Clinical_trials / Diagnostic_studies Límite: Adolescent / Adult / Female / Humans / Male Idioma: En Revista: J Nutr Biochem Asunto de la revista: BIOQUIMICA / CIENCIAS DA NUTRICAO Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos