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Comprehensive DNA Methylation Analysis of Retrotransposons in Male Germ Cells.
Nagamori, Ippei; Kobayashi, Hisato; Shiromoto, Yusuke; Nishimura, Toru; Kuramochi-Miyagawa, Satomi; Kono, Tomohiro; Nakano, Toru.
Afiliación
  • Nagamori I; Department of Pathology, Medical School, Osaka University, Suita, Osaka 565-0871, Japan.
  • Kobayashi H; NODAI Genome Research Center, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan.
  • Shiromoto Y; Department of Pathology, Medical School, Osaka University, Suita, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan.
  • Nishimura T; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
  • Kuramochi-Miyagawa S; Department of Pathology, Medical School, Osaka University, Suita, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012, Japan.
  • Kono T; Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan.
  • Nakano T; Department of Pathology, Medical School, Osaka University, Suita, Osaka 565-0871, Japan; Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama 332-0012,
Cell Rep ; 12(10): 1541-7, 2015 Sep 08.
Article en En | MEDLINE | ID: mdl-26321633
De novo DNA methylation of retrotransposons is critical for silencing. Here, we use DNA methylation analysis to examine retrotransposons in mouse male germ cells. DNA methylation of long interspersed nuclear elements (LINEs) is dependent on piRNA, and younger LINEs exhibit greater piRNA dependence. In contrast, most long terminal repeat (LTR) retrotransposons produce lower levels of piRNAs and do not show significant piRNA dependence. The relationship between DNA methylation and corresponding piRNA expression of several LTR retrotransposons was reduced in Mili-null cells, but not Miwi2-null cells. These observations raise the possibility of piRNA-dependent DNA methylation without Miwi2. Therefore, it appears that the molecular mechanisms of the gene silencing of retrotransposons are more complicated than previously thought.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatogonias / Retroelementos / Metilación de ADN Límite: Animals Idioma: En Revista: Cell Rep Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatogonias / Retroelementos / Metilación de ADN Límite: Animals Idioma: En Revista: Cell Rep Año: 2015 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos