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Phenotypic and genotypic characterization of Brucella strains isolated from autochthonous livestock reveals the dominance of B. abortus biovar 3a in Nigeria.
Bertu, Wilson J; Ducrotoy, Marie J; Muñoz, Pilar M; Mick, Virginie; Zúñiga-Ripa, Amaia; Bryssinckx, Ward; Kwaga, Jacob K P; Kabir, Junaid; Welburn, Susan C; Moriyón, Ignacio; Ocholi, Reuben A.
Afiliación
  • Bertu WJ; Brucellosis Research Unit, National Veterinary Research Institute, Vom 930001, Plateau State, Nigeria.
  • Ducrotoy MJ; Division of Infection and Pathway, School of Biomedical Sciences, College of Medicine and Veterinary Medicine, The University of Edinburgh, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK.
  • Muñoz PM; Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria (CITA), del Gobierno de Aragón, Av. Montañana 930, 50059 Zaragoza, Spain.
  • Mick V; Paris-Est University/Anses, EU/OIE/FAO & National Reference Laboratory for Brucellosis, Animal Health Laboratory, 14 rue Pierre et Marie Curie, 94701 Maisons-Alfort Cedex, France.
  • Zúñiga-Ripa A; IDISNA-Instituto de Salud Tropical y Depto., Microbiología y Parasitología, Universidad de Navarra, Edificio de Investigación, c/Irunlarrea 1, 31008 Pamplona, Spain.
  • Bryssinckx W; Avia-GIS, Risschotlei 33, BE-2980 Zoersel, Belgium.
  • Kwaga JK; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, PMB 1096, Ahmadu Bello University, Zaria, Nigeria.
  • Kabir J; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, PMB 1096, Ahmadu Bello University, Zaria, Nigeria.
  • Welburn SC; Division of Infection and Pathway, School of Biomedical Sciences, College of Medicine and Veterinary Medicine, The University of Edinburgh, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK.
  • Moriyón I; IDISNA-Instituto de Salud Tropical y Depto., Microbiología y Parasitología, Universidad de Navarra, Edificio de Investigación, c/Irunlarrea 1, 31008 Pamplona, Spain. Electronic address: imoriyon@unav.es.
  • Ocholi RA; Division of Infection and Pathway, School of Biomedical Sciences, College of Medicine and Veterinary Medicine, The University of Edinburgh, Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK. Electronic address: rbocholi@yahoo.com.
Vet Microbiol ; 180(1-2): 103-8, 2015 Oct 22.
Article en En | MEDLINE | ID: mdl-26315770
Brucellosis is a worldwide widespread zoonosis caused by bacteria of the genus Brucella. Control of this disease in a given area requires an understanding of the Brucella species circulating in livestock and humans. However, because of the difficulties intrinsic to Brucella isolation and typing, such data are scarce for resource-poor areas. The paucity of bacteriological data and the consequent imperfect epidemiological picture are particularly critical for Sahelian and Sub-Sahara African countries. Here, we report on the characterization of 34 isolates collected between 1976 and 2012 from cattle, sheep and horses in Nigeria. All isolates were identified as Brucella abortus by Bruce-ladder PCR and assigned to biovar 3 by conventional typing. Further analysis by enhanced AMOS-ERY PCR showed that all of them belonged to the 3a sub-biovar, and MLVA analysis grouped them in a cluster clearly distinct from that formed by European B. abortus biovar 3b strains. Nevertheless, MLVA detected heterogeneity within the Nigerian biovar 3a strains. The close genetic profiles of the isolates from cattle, sheep and horses, suggest that, at least in some parts of Nigeria, biovar 3a circulates among animal species that are not the preferential hosts of B. abortus. Consistent with previous genetic analyses of 7 strains from Ivory Cost, Gambia and Togo, the analysis of these 34 Nigerian strains supports the hypothesis that the B. abortus biovar 3a lineage is dominant in West African countries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brucella abortus / Brucelosis / Ganado / Genotipo Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Vet Microbiol Año: 2015 Tipo del documento: Article País de afiliación: Nigeria Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Brucella abortus / Brucelosis / Ganado / Genotipo Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Vet Microbiol Año: 2015 Tipo del documento: Article País de afiliación: Nigeria Pais de publicación: Países Bajos