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Discovery of the Once-Weekly Glucagon-Like Peptide-1 (GLP-1) Analogue Semaglutide.
Lau, Jesper; Bloch, Paw; Schäffer, Lauge; Pettersson, Ingrid; Spetzler, Jane; Kofoed, Jacob; Madsen, Kjeld; Knudsen, Lotte Bjerre; McGuire, James; Steensgaard, Dorte Bjerre; Strauss, Holger Martin; Gram, Dorte X; Knudsen, Sanne Møller; Nielsen, Flemming Seier; Thygesen, Peter; Reedtz-Runge, Steffen; Kruse, Thomas.
Afiliación
  • Lau J; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Bloch P; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Schäffer L; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Pettersson I; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Spetzler J; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Kofoed J; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Madsen K; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Knudsen LB; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • McGuire J; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Steensgaard DB; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Strauss HM; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Gram DX; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Knudsen SM; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Nielsen FS; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Thygesen P; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Reedtz-Runge S; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
  • Kruse T; Global Research, Novo Nordisk A/S , Novo Nordisk Park, DK-2760 Måløv, Denmark.
J Med Chem ; 58(18): 7370-80, 2015 Sep 24.
Article en En | MEDLINE | ID: mdl-26308095
Liraglutide is an acylated glucagon-like peptide-1 (GLP-1) analogue that binds to serum albumin in vivo and is approved for once-daily treatment of diabetes as well as obesity. The aim of the present studies was to design a once weekly GLP-1 analogue by increasing albumin affinity and secure full stability against metabolic degradation. The fatty acid moiety and the linking chemistry to GLP-1 were the key features to secure high albumin affinity and GLP-1 receptor (GLP-1R) potency and in obtaining a prolonged exposure and action of the GLP-1 analogue. Semaglutide was selected as the optimal once weekly candidate. Semaglutide has two amino acid substitutions compared to human GLP-1 (Aib(8), Arg(34)) and is derivatized at lysine 26. The GLP-1R affinity of semaglutide (0.38 ± 0.06 nM) was three-fold decreased compared to liraglutide, whereas the albumin affinity was increased. The plasma half-life was 46.1 h in mini-pigs following i.v. administration, and semaglutide has an MRT of 63.6 h after s.c. dosing to mini-pigs. Semaglutide is currently in phase 3 clinical testing.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Similares al Glucagón / Péptido 1 Similar al Glucagón / Receptor del Péptido 1 Similar al Glucagón Límite: Animals / Humans / Male Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos Similares al Glucagón / Péptido 1 Similar al Glucagón / Receptor del Péptido 1 Similar al Glucagón Límite: Animals / Humans / Male Idioma: En Revista: J Med Chem Asunto de la revista: QUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos