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BMP-7 Induces Adult Human Pancreatic Exocrine-to-Endocrine Conversion.
Klein, Dagmar; Álvarez-Cubela, Silvia; Lanzoni, Giacomo; Vargas, Nancy; Prabakar, Kamalaveni R; Boulina, Maria; Ricordi, Camillo; Inverardi, Luca; Pastori, Ricardo L; Domínguez-Bendala, Juan.
Afiliación
  • Klein D; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL.
  • Álvarez-Cubela S; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL.
  • Lanzoni G; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL.
  • Vargas N; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL.
  • Prabakar KR; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL.
  • Boulina M; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL.
  • Ricordi C; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, FL Department of Biomedical Engine
  • Inverardi L; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, FL Department of Medicine, Miller School of Medicine, University of Miami, Miami, FL.
  • Pastori RL; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, FL Department of Medicine, Miller School of Medicine, University of Miami, Miami, FL rpastori@med.miami.edu jdoming
  • Domínguez-Bendala J; Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL Department of Cell Biology and Anatomy, Miller School of Medicine, University of Miami, Miami, FL rpastori@med.miami.edu jdominguez2
Diabetes ; 64(12): 4123-34, 2015 Dec.
Article en En | MEDLINE | ID: mdl-26307584
The exocrine pancreas can give rise to endocrine insulin-producing cells upon ectopic expression of key transcription factors. However, the need for genetic manipulation remains a translational hurdle for diabetes therapy. Here we report the conversion of adult human nonendocrine pancreatic tissue into endocrine cell types by exposure to bone morphogenetic protein 7. The use of this U.S. Food and Drug Administration-approved agent, without any genetic manipulation, results in the neogenesis of clusters that exhibit high insulin content and glucose responsiveness both in vitro and in vivo. In vitro lineage tracing confirmed that BMP-7-induced insulin-expressing cells arise mainly from extrainsular PDX-1(+), carbonic anhydrase II(-) (mature ductal), elastase 3a (acinar)(-) , and insulin(-) subpopulations. The nongenetic conversion of human pancreatic exocrine cells to endocrine cells is novel and represents a safer and simpler alternative to genetic reprogramming.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Páncreas Exocrino / Diabetes Mellitus Experimental / Células Secretoras de Insulina / Transdiferenciación Celular / Proteína Morfogenética Ósea 7 Límite: Animals / Humans / Male Idioma: En Revista: Diabetes Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Páncreas Exocrino / Diabetes Mellitus Experimental / Células Secretoras de Insulina / Transdiferenciación Celular / Proteína Morfogenética Ósea 7 Límite: Animals / Humans / Male Idioma: En Revista: Diabetes Año: 2015 Tipo del documento: Article Pais de publicación: Estados Unidos