Automated N-glycan profiling of a mutant Trypanosoma rangeli sialidase expressed in Pichia pastoris, using tandem mass spectrometry and bioinformatics.

Li, Haiying; Rasmussen, Morten I; Larsen, Martin R; Guo, Yao; Jers, Carsten; Palmisano, Giuseppe; Mikkelsen, Jørn D; Kirpekar, Finn

Li, Haiying; Rasmussen, Morten I; Larsen, Martin R; Guo, Yao; Jers, Carsten; Palmisano, Giuseppe; Mikkelsen, Jørn D; Kirpekar, Finn.

Afiliación

Li H; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Rasmussen MI; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Larsen MR; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Guo Y; Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, 2800 Kgs, Lyngby, Denmark.
Jers C; Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, 2800 Kgs, Lyngby, Denmark.
Palmisano G; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Mikkelsen JD; Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 229, 2800 Kgs, Lyngby, Denmark.
Kirpekar F; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark f.kir@bmb.sdu.dk.

Glycobiology ; 25(12): 1350-61, 2015 Dec.

Article en En | MEDLINE | ID: mdl-26306636

Asunto(s)

Mutación; Neuraminidasa/metabolismo; Procesamiento Proteico-Postraduccional; Proteínas Protozoarias/metabolismo; Trypanosoma rangeli/enzimología; Glicosilación; Leche Humana/química; Neuraminidasa/química; Neuraminidasa/genética; Pichia/genética; Pichia/metabolismo; Polisacáridos/metabolismo; Proteínas Protozoarias/química; Proteínas Protozoarias/genética; Proteínas Recombinantes; Trypanosoma rangeli/genética

Palabras clave

N-glycan; P. pastoris; T. rangeli sialidase mutant Tr7; mass spectrometry; trans-sialidase activity

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Protozoarias / Procesamiento Proteico-Postraduccional / Trypanosoma rangeli / Mutación / Neuraminidasa Idioma: En Revista: Glycobiology Asunto de la revista: BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido

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