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Improvement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renale.
Pedrassani, Daniela; do Nascimento, Adjair Antonio; André, Marcos Rogério; Machado, Rosangela Zacarias.
Afiliación
  • Pedrassani D; Department of Veterinary Medicine, Universidade do Contestado (UnC), Rua Roberto Ehlke 86, CEP 89460-000 Canoinhas, SC, Brazil. Electronic address: daniela@unc.br.
  • do Nascimento AA; Department of Preventive Veterinary Medicine and Animal Reproduction, School of Agrarian and Veterinary Sciences (FCAV), Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900, Jaboticabal, SP, Brazil. Electronic address: nascimento.adjair@gmail.com.
  • André MR; Department of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil. Electronic address: marcos_andre@fcav.unesp.br.
  • Machado RZ; Department of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil. Electronic address: zacarias@fcav.unesp.br.
Vet Parasitol ; 212(3-4): 435-8, 2015 Sep 15.
Article en En | MEDLINE | ID: mdl-26299933
An available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-Dioctophyma renale antibodies in the sera of dogs using, detection of parasite eggs in urine sediment as a reference test. ELISA uses a soluble antigenic preparation of esophagus of D. renale and the optimal dilutions of the antigen, serum and conjugate were determined by means of checker board titration, using positive (n=13) and negative (n=27) reference serum. The specificity and sensitivity of the ELISA were 93.8% and 92.3% respectively and the kappa index was good (0.76). These results suggest that ELISA described may prove to be an effective serological test for detecting dogs infected and exposed to this parasite mainly dogs that are not eliminating parasite eggs through their urine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayo de Inmunoadsorción Enzimática / Anticuerpos Antihelmínticos / Infecciones por Enoplida / Dioctophymatoidea / Enfermedades de los Perros Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet Parasitol Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayo de Inmunoadsorción Enzimática / Anticuerpos Antihelmínticos / Infecciones por Enoplida / Dioctophymatoidea / Enfermedades de los Perros Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet Parasitol Año: 2015 Tipo del documento: Article Pais de publicación: Países Bajos