Your browser doesn't support javascript.
loading
Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA.
Glushakova, Lyudmyla G; Sharma, Nidhi; Hoshika, Shuichi; Bradley, Andrea C; Bradley, Kevin M; Yang, Zunyi; Benner, Steven A.
Afiliación
  • Glushakova LG; Firebird Biomolecular Sciences, Alachua, FL 32615, USA.
  • Sharma N; Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA.
  • Hoshika S; Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA.
  • Bradley AC; Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA.
  • Bradley KM; Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA.
  • Yang Z; Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA.
  • Benner SA; Firebird Biomolecular Sciences, Alachua, FL 32615, USA; Foundation for Applied Molecular Evolution (FfAME), Alachua, FL 32615, USA. Electronic address: sbenner@ffame.org.
Anal Biochem ; 489: 62-72, 2015 Nov 15.
Article en En | MEDLINE | ID: mdl-26299645
Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients, to guide therapy. They commonly use polymerase chain reaction (PCR) and reverse transcription PCR. Although these all have rapid turnaround, they are expensive to run. Multiplexing would allow their cost to be spread over multiple targets, but often only with lower sensitivity and accuracy, noise, false positives, and false negatives; these arise by interactions between the multiple nucleic acid primers and probes in a multiplexed kit. Here we offer a multiplexed assay for a panel of respiratory viruses that mitigates these problems by combining several nucleic acid analogs from the emerging field of synthetic biology: (i) self-avoiding molecular recognition systems (SAMRSs), which facilitate multiplexing, and (ii) artificially expanded genetic information systems (AEGISs), which enable low-noise PCR. These are supplemented by "transliteration" technology, which converts standard nucleotides in a target to AEGIS nucleotides in a product, improving hybridization. The combination supports a multiplexed Luminex-based respiratory panel that potentially differentiates influenza viruses A and B, respiratory syncytial virus, severe acute respiratory syndrome coronavirus (SARS), and Middle East respiratory syndrome (MERS) coronavirus, detecting as few as 10 MERS virions in a 20-µl sample.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orthomyxoviridae / Virus Sincitiales Respiratorios / Infecciones del Sistema Respiratorio / ARN Viral / Coronaviridae / Tipificación Molecular Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Biochem Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orthomyxoviridae / Virus Sincitiales Respiratorios / Infecciones del Sistema Respiratorio / ARN Viral / Coronaviridae / Tipificación Molecular Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Biochem Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos