Atractylenolide I protects mice from lipopolysaccharide-induced acute lung injury.
Eur J Pharmacol
; 765: 94-9, 2015 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-26297303
Atractylenolide I (AO-I), one of the major bioactive components isolated from Rhizoma Atractylodes macrocephala, has been reported to have anti-inflammatory effects. In the present study, we investigated the protective effects of AO-I on acute lung injury (ALI) using LPS-induced ALI mouse model. Lung injury was assessed by histological study. Inflammatory cytokines TNF-α, IL-6 and IL-1ß production were detected by ELISA. TLR4 expression and NF-κB activation were measured by western blot analysis. The results showed that treatment of AO-I significantly attenuated LPS-induced lung wet-to-dry weight ratio and MPO activity. Meanwhile, treatment of AO-I significantly inhibited the production of TNF-α, IL-6, IL-1ß, IL-13, and MIF production in bronchoalveolar lavage fluid (BALF), as well as neutrophils and macrophages in BALF. AO-1 could up-regulate the production of IL-10 in BALF. Besides, LPS-induced TLR4 expression and NF-κB activation were suppressed by treatment of AO-I. In conclusion, the current study suggested that AO-I protected mice acute lung injury induced by LPS via inhibition of TLR4 expression and NF-κB activation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sesquiterpenos
/
Lipopolisacáridos
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Mediadores de Inflamación
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Lesión Pulmonar Aguda
/
Lactonas
Límite:
Animals
Idioma:
En
Revista:
Eur J Pharmacol
Año:
2015
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos