Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors.
J Vet Med Sci
; 78(1): 133-8, 2016 Jan.
Article
en En
| MEDLINE
| ID: mdl-26290128
Two commercial PRRSV ELISA kits (IDEXX and Bionote) were evaluated for their sensitivity and specificity using 476 PRRS-positive serum samples collected from 7 animal challenge experiments and 1,000 PRRS-negative sera. Both ELISA kits exhibited 100% sensitivity with sera collected 14 to 42 days post-infection, and the results from the kits were highly correlated (R(2)=0.9207). The specificity of IDEXX or Bionote kit was 99.9% or 99.7%, respectively. In addition, the Bionote ELISA kit was used to examine 100 sera that were determined to be falsely positive either by IDEXX 2XR or 3XR ELISA, and only 7 of these samples were found to be positive. These results indicate that both ELISA kits exhibited similar levels of sensitivity and specificity and would complement one another for the verification of false-positive samples.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Virus del Síndrome Respiratorio y Reproductivo Porcino
/
Síndrome Respiratorio y de la Reproducción Porcina
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
J Vet Med Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2016
Tipo del documento:
Article
Pais de publicación:
Japón